How to convert a domestic microwave assisted synthesis for dedicated instrumentation?

Hello, I just dived into the topic of microwave assisted synthesis and found a literature I would like to reproduce. The problem is in the literature, a domestic microwave oven is used which we...

27 January 2021 4,788 4 View

What sensors are typically placed on aircraft, especially experimental?

In addition to sensors such as load cells, prandtl tubes, pitot tubes, which other sensors are put on experimental aircraft

19 November 2020 6,945 3 View

How best can i obtain the 'P' parameter for a PID controller to be used on tiltwing UAV?

For the tiltwing UAV which i am currently designing, i am going to use a PID controller which i will develop in MATLAB. I want to determine the most optimum value for the 'P' parameter...

01 November 2020 7,324 7 View

How do I solve the warning "Excessive distortion at a total of 6 integration points in solid (continuum) elements" (Abaqus)?

Hi, I simulate a nano - 2D indentation process in Abaqus 6.16. The simulation is in Abaqus/Standard and non-linear is switched on. The process is force controlled - I typ in the force and want the...

28 April 2018 925 5 View

Does anyone have experience with the ABI StepOne Real-Time PCR system?

10 September 2014 4,651 4 View

What is the most reliable and affordable technique for transcript profiling eukaryotic plants?

17 July 2014 8,821 2 View

What is virus titer/concentration?

I would like to study the interaction between cassava viruses and a sort of cassava genotypes. I would like to do this by measuring virus concentration. Is it right to call this virus...

19 June 2014 2,170 10 View

Can I use in vitro transcription of cloned cDNA to generate RNA for calibration in a qRT-PCR?

I would like to perform absolute quantification of RNA viruses in plant tissue and was wondering if approaching this through in vitro transcription makes sense.

26 January 2014 6,320 5 View

Is there a possibility to get involved in this project: Neuroeconomics?

I am from South Africa..Masters' student in Economics, also employed at the Department of Economics at University of South Africa. My studies focus on Neuroeconomics. It is a very interesting...

01 January 1970 4,273 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Is there a fast method matching citations to bibliography (manually typed references)?

I need to be able to match in-text citations to a reference list and back again in very large documents (100+ pages) WITHOUT the use of referencing software like Endnote. my method is to type the...

27 February 2021 9,848 1 View

Can impedance of antenna be less than 50 ohms?

I am simulating helix antenna in hfss, right now, giving excitation with 50 ohms port. When I see the results of Z parameter (real). It shows lower than 50 ohms. Do not understand, if it can be...

27 February 2021 4,218 7 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View