Rudolph Shirima

5 Questions 4 Answers 0 Followers

Questions related from Rudolph Shirima

I am looking for a suitable Sanger Sequencing service provider that gives best quality results and yet affordably cheap. Any suggestions?

Moderately cheap Sanger sequencing facility.

28 June 2017 4,183 0 View

Does anyone have experience with the ABI StepOne Real-Time PCR system?

I am setting up a new StepOne Real-Time PCR system (48 well) that came with a laptop computer with all the installation completed. The RNase P Experiment "Failed". There were virtually no...

11 September 2014 4,745 4 View

What is the most reliable and affordable technique for transcript profiling eukaryotic plants?

I would like to monitor gene expression in virus infected plants. Is there any cost effective but robust method for this?

18 July 2014 8,908 2 View

What is virus titer/concentration?

I would like to study the interaction between cassava viruses and a sort of cassava genotypes. I would like to do this by measuring virus concentration. Is it right to call this virus...

20 June 2014 2,266 10 View

Can I use in vitro transcription of cloned cDNA to generate RNA for calibration in a qRT-PCR?

I would like to perform absolute quantification of RNA viruses in plant tissue and was wondering if approaching this through in vitro transcription makes sense.

27 January 2014 6,420 5 View