My protein is in SEC buffer (50mM Tris pH:9.0, 1mM EDTA, 150mM NaCl, 0.8mM (NH4)2SO4 ). I have made automatically two (20mg/ml and 40mg/ml) setting drop screens by hampton kits which almost leads to some kind of Gelatinous Precipitant. In manually hanging drop all of the screens (clear or precipitated drops) will make skin !! How can overcome this situation?
Also in some condition (2-2.4M Na malonate pH 8-9) and (60% Tacsimate pH 8.0) it will make some different kind of skin which is hard!! this kind of skin like a crystal will crush by using seeding tool (2 last figure)!!
Probably this can be useful: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3106348/
for the last 2 it's sulfate salts
Actually by my personal experience it means that your protein is crushing in the drops. You can try increase protein solubility adding ligands if they are known or some common compounds which can increase solubility like KSCN, glycerol and so on (N.B. add them to the protein solution before screening).
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