17 Questions 14 Answers 0 Followers
Questions related from Zaiddodine Pashandi
I am docking multiple ligands (new designed ligand) against my protein using Autodock Vina in Chimera. The results displayed in this program are somewhat strange, because in some cases the ligands...
17 January 2024 9,642 3 View
I used a KOD DNA polymerase for PCR that works very well. Then I used this enzyme to run a TaqMan probe real-time PCR but the results waere unexpected. I attached the results. The fluorescence...
31 December 2022 6,537 4 View
I am going to fabricate a free flow electrophoresis (FFE) chamber. The chamber will contains the sample plus buffers (electrolytes). In capillary electrophoresis, IEF or gel-based electrophoresis...
23 June 2019 9,505 3 View
what are the means and differences of "< |M|\S2\N >" , "< |M| >\S2\N" , "< |M|\S2\N > - < |M| >\S2\N" and "< |M| >\S2\N / < |M|\S2\N >" in .xvg file of...
15 May 2017 6,520 1 View
According to my chemistry knowledge, The only thing that changes an equilibrium constant of a specific reaction is a change of temperature. However, I have seen in some literature which is...
15 August 2016 6,241 7 View
According to the companies Description, BAPTA is Ca2+ chelator exhibiting a 105-fold greater affinity for Ca2+ (Kd = 110 nM) than for Mg2+. After BAPTA complexes with Ca2+, the UV spectrum shifts...
21 June 2016 5,924 5 View
I have sent my crystal for synchrotron and now I have diffraction images. I want to start the data processing. It is my first time and I need to know the step by step protocols (for MR or SeMet)...
07 March 2016 510 3 View
My protein is in SEC buffer (50mM Tris pH:9.0, 1mM EDTA, 150mM NaCl, 0.8mM (NH4)2SO4 ). I have made automatically two (20mg/ml and 40mg/ml) setting drop screens by hampton kits which almost leads...
12 February 2016 807 2 View
I want to make a cryoprotectant solution for my protein crystal. I don't have many crystal to check several cryogenic conditions. My crystallization buffer contain: 50% MPD, 0.1M Tris pH 8.6, 0.1M...
14 December 2015 8,943 6 View
What is the basic theory of peak shift (Blue or Red) in circular dichroism spectrum of proteins (208,222 nm of alfa-helix or 218nm of betta sheet). and what is the mean of this blue/red shifts?...
14 September 2015 4,202 4 View
What is true about d in bragg's law? please explain. 1- The d is distance of bond (covalent or electrostatic) between two atoms which are located in adjacent bragg's planes and is in angstrom...
26 January 2015 8,039 4 View
I want to crystalize my protein with its ligand (Mw 423.5 gr/mol which is in the range of crystal staining dyes). Is it obligatory step separation the charged proteins (Pr+ligand) from uncharged...
05 January 2015 1,492 7 View
In several screens, I saw some crystals that look like protein crystals, but they redissolved and became unapparent during checking them under light microscopy after a few seconds. For example, in...
09 December 2014 8,657 7 View
I applied several screening buffers for crystallization of my proteins. in three of them I have seen three different shape crystals (plate like, rod, triangular star like!) that seems to been my...
09 December 2014 4,791 5 View
How can improve a gelatinous precipitated (GP) and oil or spherulites in screens of protein crystallization? which will have the most effect changing the buffer (pH), salts or precipitant? I...
20 October 2014 2,033 6 View
How I could determine the extinction coefficient (Molar absorptivity) of my protein experimentaly? Is it okey if I calculate the slop of Absorption vs Concentration? for this I must prepare some...
27 August 2014 8,630 5 View
I have accessibility to a cheap source of plexiglass scraps. I am thinking to a recycling projects and producing new products based on PMMA recycling. What is your suggestion for PMMA recycling?...
01 January 1970 8,794 7 View
