what is the wavelength for max absorption of 1-butylamine, using quartz cuvette and UV-Vis
I am trying to measured butylamine concentration in at a range of 1-150 mg/L in water solution with 0.1M NaNO3 and 0.01M Ca(NO3)2. I am having problem with finding the wave length for max absorption
Thanks
I hope these articles will be helpful to you.
https://books.google.com.pk/books?id=mVOoAdKxWsYC&pg=PA47&lpg=PA47&dq=butylamine+wavelength&source=bl&ots=5TubivBx-L&sig=eBHbj83IdTRj-9sEffUnf3l1PDU&hl=en&sa=X&sqi=2&ved=0ahUKEwilwYm_n_7JAhUDTBQKHfjLC2EQ6AEIHzAB#v=onepage&q=butylamine%20wavelength&f=false
http://www.rsc.org/suppdata/cc/c0/c0cc04942e/c0cc04942e.pdf
http://scholarsresearchlibrary.com/aasr-vol4-iss1/AASR-2012-4-1-150-154.pdf
Dear Mr. Alon
You just chose one of all solution between 1- 150 ppm (mg/L). Then, measure the solution on visible and unvisible wavelength range (200-900 nm).
Warm regards
Ganjar
Alon, you will have high background absorption due to nitrate ions present. Butyl amine band would correspond to n-sigma* transition in the UV region, approximately around 210 nm. I will not recommend such quantitation.
Take a baseline correction with your blank. Then take a sample scan from 190 to 900 nm (covering both UV and Visible region). This will enable you to find out the wavelength of maximum absorption.
All aliphatic amines have very low absorption-coefficients e.g. methylamine 220nm
What do you use as a blank? This compound should show an absorption maxima around 200 nm. It is necessary to remove the influence of NaNO3 and Ca (NO3) 2 of a blank which is to contain these compounds in the same concentration and in the same solvent at the same pH value at which is in the sample. Then you should record absorption spectrum in 190-800 nm. If you still have problems, try changing the concentration of the sample.
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