Usually, we perform SDS-Page in acrylamide gel and we use SDS buffer. If I use 1x TBE buffers for DNA or PCR products for running in acrylamide gel what will be the power/running condition?
between 1 and 8 volts per cm (length of gel). 1v/cm maximum resolution slowest speed, 8v/cm fastest speed, lower resolution, everything in between is an acceptable compromise. I run at 35v (mini-gel size).
between 1 and 8 volts per cm (length of gel). 1v/cm maximum resolution slowest speed, 8v/cm fastest speed, lower resolution, everything in between is an acceptable compromise. I run at 35v (mini-gel size).
Thank you very much Nathan Sweeney. I also running at 35v. Would you please inform me the corresponding ampere of 35v.
You cannot control everything. Potential and current are bound together with the resistance of your gel (which you can assume a constant).
Thank you Jiri Koubek. I would like to maintain constant Potential.
Run the gel either at a constant current or at a constant voltage. Its better to use 1X TAE buffer for your DNA samples. For small agarose gels, one can use 80V.
hi Mohammad Safiqul Islam
in agarose gel electrophoresis should be constat the amper on 140
voltage =100
in PAGE gel the mili amper should be on 45-60 and the voltage set on 90
i run my gels based on uper condition and have been good results
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