Is it possible to check the gene expression with a conventional PCR machine?

After extracting RNA, we can get cDNA by reverse transcriptase reaction with a conventional PCR. Then we can amplify the target gene and compare the PCR products with the house keeping gene...

01 February 2018 4,963 9 View

How can you calculate the sample size, statistical power, effect and sensitivity of a Pharmacogenomics adverse drug reaction study?

In case of adverse drug reaction study, there is no control group and only the patients use the drug. After using the drug, some patients show some adverse drug reactions and others do not show...

05 June 2015 7,039 6 View

How can I calculate the sample size and sensitivity for a pharmacogenomics case control study?

04 May 2015 2,210 5 View

What is the voltage or ampere for running DNA or PCR products in acrylamide gel?

Usually, we perform SDS-Page in acrylamide gel and we use SDS buffer. If I use 1x TBE buffers for DNA or PCR products for running in acrylamide gel what will be the power/running condition?

11 December 2014 6,866 8 View

What is the exact rule of assigning impact factor to a journal?

Some journals are sending mails for inviting articles showing their impact factors before publishing their initial issues or after publishing several issues. Is it the authentic impact factors?...

06 July 2014 4,081 2 View

Is SSRN e-Journal Scopus indexed?

I have publication in SSRN. I want to know whether it is Scopus indexed? Does it show impact factor? What is its ISSN No.?

02 March 2021 4,565 3 View

What is the most reliable source to get Impact factor of journals?

Source to get Impact factor of journals

01 March 2021 6,654 2 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

Is IMPACT FACTOR justified in case of Taxonomy?

There is huge pressure of getting good impact factor on scholars now a days. On the other hand Taxonomy is a very dry subject and the concerned papers can only get citation in same group of organism.

26 February 2021 5,851 1 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

Am i able to conduct a factor analysis on each of my subscales, as opposed to the whole scale?

My psychometric scale contains 38 items in total, with 4 subscales (20, 6, 6 & 6 items respectively? Can i run a factor analysis for each subscale rather than the scale as a whole for the...

24 February 2021 8,856 8 View

What are alternatives to factor analysis for a small sample?

My sample size is too small to conduct a factor analysis. I created my own psychometric scale for measuring Effectiveness of Support, which contains 38 items (4 sub-scales) Im really struggling...

23 February 2021 9,287 12 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View

How to find orthotropic material properties?

I am doing an impact analysis of bullet on a plate. But for that simulation, I require orthotropic properties of materials like PPS and PEEK which are Unidirectional type. How to find them?...

16 February 2021 8,775 2 View

Which Journals would you advise to submit a Letter which briefly talks about neurosurgical treatment of neuro-oncological patient during Sars-COV-2?

Possibily not an open access journal which does not add any publishing costs once being accepted. Thank you in advance

09 February 2021 717 6 View