What is the usual yield of DNA (PCR product) after Dpn1 digestion?

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Domenico Sanfelice

Dpn1 only cuts bacterial DNA (methylated) , it does not digest the amplified (newly synthesised) DNA. Probably something went wrong in the PCR.

Anthony Han Kang Tee

how much concentration u used for pcr amplification?

the reduction of concentration could actually was the digestion of your template DNA. To my understanding DpnI should have not much effect on amplicon yield

Dominique Liger

Hi there,

You may reamplify the plasmid by repeating the PCR step on the DpnI digested product. Anyway you don't need a lot for transformation.