Pradipta Patra

4 Questions 3 Answers 0 Followers

Questions related from Pradipta Patra

Sample molecule in HPLC are retaining in the next consecutive run. How is it happening?

I am using Agilent 1200 series HPLC to measure Xylose with the Aminex 87H column using 5mM H2SO4. When I am injecting Xylose standard,molecule is not retaining. No peak is there. In the next run...

07 July 2019 2,625 9 View

What is the usual yield of DNA (PCR product) after Dpn1 digestion?

I amplified a 11KB plasmid backbone (leaving some base pairs ). I did the gel purification with a yield of 85ng/MicroL in 15MicroL elution. After Dpn1 digestion it Came to 15ng/MicroL. [I...

04 April 2018 6,301 3 View

When should I use HPLC purified oligo?

Hello, I am preparing donor DNA for cloning using CRISPR-Cas9 method. When I am attaching the overhangs of 60neucleotides in two PCR programs (30ntds+30ntds). When I am doing 2nd PCR (Touch Up,...

02 February 2018 7,646 3 View

How long a working plasmid aliquot can be used? in other way, how long is it stable [After use I store it in -20'C] ?

I have eluted the Plasmid from transformed bacteria with 500ng concentration. How long it can be used? (Based on quality)

11 November 2017 6,486 1 View