How do I prevent cell loss during wash/fix/mount of rat lymphocytes?

I am culturing rat lymphocytes from whole rat blood, then washing in PBS followed by fixing in Carnoy's fixative. At this point I am mounting cells on a slide. Somewhere in this process I have...

20 January 2020 2,571 2 View

Rare Sugars in Food?

Dear all, recently I was confronted with the statement that "rare sugars" can be used to identify food fraud or the use of enzymatic food treatment. Initially, I was surprised to learn, that...

17 September 2018 9,202 5 View

Plant gall nomenclature?

Hello, is there a nomenclature for plant galls (induced by parasites) which is generally accepted?

21 March 2018 1,509 3 View

Is this mycoplasma?

Dear Scientific Community :) A colleague recently tested his melanoma (ME1402) cells for mycoplasma and obtained some strange images of rod-like structures - quite different from the 'dot-like'...

14 December 2016 4,549 3 View

Can you TA clone an insert into pGEM T easy after designing primers with restriction sites?

Good day fellow researchers, I have recently designed primers with restriction sites which I am going to use to amplify my insert and clone into a reporter vector. After reading up on TA cloning I...

06 November 2016 3,733 10 View

Does anyone knows what kind of polyeder these copper and lead models represent?

I got them from a mineral collector, but obviously these are no crystal models.

04 October 2016 8,779 5 View

Can anybody recommend a good antibody against Jun for WB and ChIP in rat?

Can anybody recommend a good antibody against Jun for WB and ChIP in rat? Thanks.

06 June 2016 4,247 2 View

Why do I obtain a strong Z-conrast in HAADF-STEM images?

What is the physical reason for the Z-contrast in HAADF-STEM images?

23 May 2016 4,675 3 View

Does anyone have advice on how to optimally culture and maintain leukemia cells in suspension?

24 January 2016 4,301 3 View

Can you help me to understand molecular combing?

Hi colleagues! I need your help, because I have to understand the molecular combing method. I want to use the Molecular Combing system from Genomic Vision. Unfortunately, after reading several...

23 July 2015 4,229 5 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Could you please share your experience with me on the type of 96 wells plate for MIC determination: Treated Vs Untreated and Round-Vs Flat-bottomed?

I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...

17 February 2021 7,029 3 View

Glucose 6 phosphate dehydrogenase deficiency & NSAIDs - is this combination harmless ?

G6PD deficiency is quite common in our anesthesia practice in the middle east. Intraop/postop analgesia in them is an issue here. Many websites/books give contrasting advise with former advocating...

10 February 2021 4,817 3 View

Is this grounds for theophylline toxicity even though her plasma is within the therapeutic range? if it is, how is that possible?

A patient was given 300 mg Theophylline taken twice a day for the treatment of her bronchial asthma and has a positive clinical response. She has been compliant in taking her medication however,...

09 February 2021 3,489 3 View

I am looking for a postdoc. Can anyone guide me in this respect?

My PhD thesis entitled as "Anticarcinogenic activity of Terpenoid containing plants against induced Hepatocellular Carcinoma (HCC). My extensive experimental work critically examined the potential...

08 February 2021 8,628 3 View

SARS-CoV-2 Spike Protein Western Bands?

Hello, I am western blotting SARS-CoV-2 spike protein from lentiviral using an antibody against S1. To prepare samples, I mixed concentrated lentivirus 1:1 with 2X Laemmli buffer and boiled...

07 February 2021 3,643 4 View