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Questions related from Pedro Elias Marques
We noticed that our HepG2 cells have been growing in large clumps lately. Cells have been cultured in DMEM plus FBS and trypsinized according to regular protocols. They don't seem to be...
28 April 2016 270 8 View
I need an easy and straightforward way to measure leukocyte degranulation, mostly neutrophils in vitro. I have considered side scatter and myeloperoxidase substrate reaction, but it didn't work...
12 November 2013 9,520 3 View
I need fluorescent markers for the liver endothelial cells. I have used anti-Pecam antibodies and they worked well, but something else that shows endothelial cell function and health would be great.
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2 fluorophores (e.g. green and red emission) one 2-photon laser wavelength (~ 900 nm).
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I want to fluorescently label tissues for intravital microscopy, in an unspecific manner. I have used Rhodamine 6G, i.v., and it worked pretty well but I need one in another wavelength.
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