What is the (Trade-off) best way to allign microscopy images? Elastix/Ant? Or skimage-register like?

More Alessandro Crimi's questions See All

Can someone explain me how to insert the actual distance between two well logs in Petrel (picture attached)?

I uploaded two well logs to the "Well section window" of Petrel and they were attached together without any space. Then, I modified the horizontal scale from "relative" to "constant (50)". They...

12 June 2024 8,666 2 View

DNA purification from ATCC 25922 E.Coli strain with QIAGEN kits?

Hi everyone, We've got a plasmid we want to purify from E.Coli strain ATCC 25922. We usually use QIAGEN plasmid purification kits but we've never purified DNA from this particular strain. Would...

20 May 2024 3,803 1 View

steam distillations with urea/water solutions. do you have any directions to give me?

Good morning, I am carrying out steam distillations with urea/water solutions on fresh rosemary, urea has a relatively low vapor pressure and in theory the vapors generated by this solution should...

30 April 2024 3,920 1 View

Glycerol stock pichia pastoris ?

Hi ,I've woken up my electroporated picha pastoris strain from the glycerol stock at -80°C.from 2020. The literature said that it could be stored for years I tried to cultivate it, and the strain...

23 April 2024 5,940 8 View

Any updates of macroeconomic variables and energy modelling being tested in econometrics?

Are there new projects and studies considering recursive approaches, in energy transition and resources, being empirically tested with regression analysis and models?

14 April 2024 7,024 4 View

FeCl3 in HCl + culture media for microbial growth?

Hello, I've seen that this question has been already asked other times on Reddit and ResearchGate but with different details. So I'm asking it too with my specifications. I need to prepare...

18 January 2024 171 0 View

How to model a packed bed in Aspen Custom Modeler?

Hi, I am modeling a packed bed in ACM. I have to write 3 differential equations. At the moment the model is under by 6, but idk how to write boundary conditions. Plus, shall the model be under by...

09 December 2023 3,527 0 View

How to interpret SIMPER p-values in R?

I am doing a SIMPER analysis (in R) with the values relating to individuals contacted in different reproductive seasons to see how the surveyed bird community has changed, and which species...

05 November 2023 1,382 0 View

Is there any deterministic phase transiton other than cellular automata's?

I'm working on a many-unit model that shows a deterministic phase transition driven by a control parameter that do not contain noise or disorder, and drives the system to chaos basically, but at...

28 September 2023 7,091 14 View

RNA extraction from dynabeads after Cross-linking immunoprecipitation for RNA-seq?

Hi everyone, I am interested in the RNA interactors of a specific protein but I am struggling a bit with the RNA extraction from the dynabeads (invitrogen) after CLIP. What I did until now was...

17 September 2023 2,256 0 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

In SEM, if a latent variable consists of three observed variables, and each observed variable has four items, what should I do?

Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...

31 July 2024 3,913 6 View

Please guide me how to prepare samples for zeiss confocal microscope?

i am working on ischemic stroke. for immunoflourescence i want to use zeiss confocal microscope.before i used VS120 but the images from vs120 not so good now i want to use zeiss confocal but i...

30 July 2024 2,349 1 View

I have synthesized a composite of ZnO and In2O3 with variations in ZnO content. In Raman , what is the peak observed at 130 cm-1?

Dear all, I have noticed, as the ZnO content increases, the Raman intensity at 100cm-1 also increases. However, there is a peak nearby at 130 cm-1, which I could not find in any paper related to...

28 July 2024 5,410 1 View

Bootstrapping in SEM Amos ?

Can a researcher use small sample size of 40 while using SEM with bootstrapping of 1000 and is it possible to get published

28 July 2024 4,402 2 View

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM images?

Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?

25 July 2024 5,482 2 View

Alessandro Crimi

Chatgpt score gave a 97% confidence that the entire text apart your greetings is generated by Chatgpt. Why would you give a very general not useful answer from Chatgpt?

Barira Maryam

I used ChatGPT for rephrasing boss, with 100% confidence. If you don't want to, just ignore it. Why do you waste your time, texting me back?

because this is a platform to discuss science and personal experience, we all can write on chatgpt, you clearly never used those things you are referring to, and for unknown reasons use chatgpt to give us pointeless answer. Please don't do it.