I need sequencing many samples of human mitochondrial DNA. I do not know how many ( concentration, amount) of DNA I should need for this.
Thnak you
How are you going to sequence the mitochondrial genome ?. Mitochondral genomes can be sequenced as part of the whole genome and assembled or from purified mitochondrial DNA. Depending on the choice the requirements will vary.
Thank you for the answer Kandasamy . I will sequence it as part of whole genome by NGS .
Hi Ana. Are you just going to sequence human genome and not use any type of capture? Because of the copy number differences between mtDNA and the human genome you should not have any problems getting enough coverage for the mtDNA. However, to see low level heteroplasty, you'll want at least 500x, preferable 1,000-fold coverage (or more). Maybe you could provide a bit more information on how much human genomic DNA you are starting with and what you are hoping to see?
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