If we seed human pluripotent stem cells (hPSCs) in absolute Matrigel and allow them to form embryoid bodies over the next ~5 days then how much time it takes for them to spontaneously differentiate when we remove the stem cell culture media and maintain them in spontaneous differentiation media? Also, I'd like to know different media for spontaneous differentiation.
Please let me know if you have any information regarding this or if you've come across any articles or know anybody in this field who can help me.
Thanks in advance!
Article Analysis of Embryoid Bodies Derived from Human Induced Pluri...
Above is an article that may give you smoe useful information.
Well, they'll BEGIN to visibly differentiate within just a few days but the process will continue for weeks. Sometimes you'll see cardiac beating within a couple weeks and others it might be well over a month. Of course it also will depend on the media used.
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