Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

How long can we consider that the data obtained with qualitative social research methods are "current"?

I cannot find any reference that deals with the temporal validity of qualitative data. I think there is a gap in the methodological literature on the temporal validity of the research results and,...

05 February 2021 9,788 20 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

What is the most adequate (serum) dilution to use to determine IgE in mice serum via ELISA assay (commercial kit)?

We have a commercial kit from RayBioTech to determine IgE from mice serum. In accordance with the supplier's instructions, we have to use a dilution of 1:10000 of the serum. Is this dilution the...

06 January 2021 4,217 3 View

Is there any reference levels of IgE in healthy mice serum, like there are for other biochemical processes/components?

My question goes for reference levels of IgE in mice serum. I know that there are tables for the most common biochemical processes/compounds (red blood cells, white blood cells, AST, ALT, etc),...

06 January 2021 8,215 3 View

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It is important for me to know the opinion from your point of view as researchers.

30 December 2020 988 2 View

Can I use the images of Collection 1 Level 2 Landsat 5 TM / Landsat 8 OLI to perform vegetation indices right away?

I'm aware that in order to perform any vegetation index like NDVI, SAVI or OSAVI you need to do some atmospheric, radiometric and terrain corrections. I'm pretty sure that Level 2 images from...

21 December 2020 4,088 3 View

No title

13 December 2020 3,861 3 View

How can I do the bang's blinding index and james blinding index?

Hi! I would like to know with which statistical software the bang blinding index and the James blinding index and how to do it. Thanks

09 December 2020 1,363 2 View

Hi all, My research questionnaire has 3 sections each having a mix of yes/no and multiple choice questions. How can I test its reliability ?

The sections are about awareness, practice, and perception regarding waste management. Thanks

20 February 2021 5,722 3 View

Is there research limitation on the relationships between digital capabilities, market orientation, brand perception and business performance?

I am looking for supporting studies discussing literature limitations on the relationships between digital capabilities, market orientation, brand perception and business performance. Also, a...

14 February 2021 7,457 5 View

How does trust influence the development of environmental projects?

Hello Scholars, I am exploring "trust" relevance in the development of socio-technical systems. What is your perception of cognitive values like trust to the energy transition/implementation of...

09 February 2021 8,133 5 View

Can you realize "top-down" and "bottom-up" ARE THE SAME THINGS at important junctures IN ONTOGENY (child development)?

Can you realize "top-down" and "bottom-up" ARE [ or certainly can, if not MUST, be ] THE SAME THINGS at important junctures IN ONTOGENY (child development)? This Question is NOT addressing YOU...

09 February 2021 5,289 1 View

How to know the threshold limit value of a sar image...??

i want to do thresholding by segregation of water areas from non water areas for that i am using model maker in ERDAS but i am unable to get the threshold limit value of my image.

26 January 2021 8,277 1 View

How to compare group means across different countries appropriately?

Let's say that I'd like to compare male respondents' and female respondents' perceptions of government across 30 countries, and test if there is statistically significant gender differences in...

19 January 2021 3,062 4 View

Inexpensive eye tracking hardware + software solution?

Hello, I am starting a project that will require an eye tracking device. I've seen eye tracking hardware + software solutions that are $10,000+ so I am hoping to find one that is significantly...

18 January 2021 8,121 12 View

What is the best Perception instrument that I could use for Student Perception towards a subject?

I am starting to write my thesis for Graduate School and I am struggling to find appropriate instrument regarding student perception instrument. The perceptions to be discussed are (a) interest...

17 January 2021 9,218 3 View

Is 30 respondent enough for two independent variables (4 level * 3 levels) in the statistical analysis?

In the case of the respondent's perception analysis, the sample size was 30 for making the perception of two factors with 4 levels and 3 levels.

12 January 2021 2,301 3 View

What statistical tool to be used in finding the significant difference between face-to-face and online learning?

I only have one sample size. I want to find if there is a significance difference between BSED-Math Students' Perceptions on Face-to-Face and Online Mathematics Learning.

06 January 2021 8,470 1 View