What is the evidence that carcinomas spread through the lymphatic system, rather than directly through the blood?

Certainly many carcinomas (epithelial derived tumors) spread at least in part through the lymphatic system since lymph node metastases exist, and also are part of clinical prognostication. That in...

11 December 2015 987 10 View

What type of cancer did Thomas Ashworth's patient suffer that gives so many Circulating Tumor Cells?

In 1869, the resident physician at Melbourne hospital, Australia, Thomas Ashworth for the first time observed cells from a postmortem blood sample, from a patient who had about 30 subcutaneous...

11 December 2013 2,528 4 View

The ideal CTC isolation instrument?

Current methods of isolating CTCs are based on either specific cell surface markers, such as EpCAM (which limits the range of cells one can isolate), on size, density or on screening massive...

06 July 2013 9,357 5 View

How do you recognise epithelial-to-mesenchymal transformed (EMT) cancer cells in clinical samples?

They should have reduced or absent epithelial characteristics, so how do you discriminate them from authentic mesenchymal cells?

06 July 2013 3,902 19 View

Is it fair to say that cancer, by and large, is a disease of loss of gene function, and that that explains the relative lack of useful biomarkers?

The loss of gene function would be expected to be close to random initially, but the cancer specific effects would occur because of hits on tumor suppressor genes. There are of course notable...

06 July 2013 2,726 1 View

Are small, regular size, or large cancer cells more malignant?

Since there is a rough biological correlation between the amount of chromosomal material and cell size, one could expect those aneuploid cells with large duplications to be both large and highly...

06 July 2013 6,637 32 View

What is the best way to assess anoikis, or apoptosis, in circulating tumor cells?

The fraction of dying cells would be expected to be of less scientifically and clinically interesting. It would be good to know the fraction and to know which are actually living and may do...

06 July 2013 5,674 2 View

What is a good animal model for quantifying metastatic yield?

To dissect the efficiency, or yield, of metastasis of different cell lines.

06 July 2013 1,708 4 View

What is the minimum hole an invading cancer cell can get through?

In vitro, and in vivo, experiments would be expected to tell us the minimal diameter an invading cancer cell can get through. It would be much smaller than the static diameter of the cell. But...

10 November 2012 5,022 3 View

Are EpCAM negative circulating tumour cells what really matters?

EpCAM positive circulating tumour cells would, by definition, retain a fair amount of epithelial characteristics. Does that mean that hey are not (very) invasive and do not have (much) stem...

10 November 2012 5,409 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View