What is the standard lift off process in EBL ?

More Shravani Anagandula's questions See All

How do I convert my canine C reactive protein readings from ng/ml to mg/l?

I obtained my readings using a canine specific ELISA kit, which measured the CRP in ng/ml. Is there a specific conversion factor to convert these readings to the SI unit mg/l? Any input is greatly...

13 April 2021 7,054 4 View

How can i prepare a biological protien sample ?

Hello all I have a sample of protiens consisting of nanofibres of 1 to 3 nanometers diameter with long length and also comprises salts like NaCl. tried with different dilution levels, but...

26 January 2020 8,390 2 View

How can I get good image of thin film of 7nm thickness on SiO2 substrate?

I have a sample containing SiO2 as a substrate ,up on that 7 nm thickness of Fe and 0.4 nm thickness of Al2O3, expected there are nanoparticles of size range of less than 100nm ,basically...

04 December 2019 3,291 7 View

Hello Dear RG members. How do I image a glass sample in FESEM ?

There are protiens and lipid samples grown on the glass .I tried pasting a carbon tape to a side and imaging but dint work out. Tried with various Acc.Voltage and probe current but didn't work...

25 November 2019 1,523 3 View

How to do 3d reconstruction from SEM images?

Hello All, I work on JSM-7200F basically dealing with thin film kind of samples and nanoparicles. so how do we 3d reconstruct from sem images? what are the softwares available for that? Is...

10 June 2019 1,256 4 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Adhesion strength of coating?

How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...

10 August 2024 942 3 View

How do I conformally do PR spin coating on trench structure?

I did PR spin coating on trench structure. I used AZ P4620 PR and the thickness or PR is around 11um. The substrate is Si. And my trench structure depth is 141um(negative way). Even though I...

08 August 2024 2,298 0 View

1. If I can quantize the atom using this hyperbolic spiral and classical physics, could nature do the same?

If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...

07 August 2024 5,343 2 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,563 0 View

How does grain and grain boundary affect the ceramic when studying its dielectric properties?

I am not able to get good literature and the physics behind how first these grains and grain boundaries arises out of no where when we make a pellet to study its dielectric properties and then how...

07 August 2024 5,177 3 View

Why do i have a weak plasma and no coating during rf sputtering?

I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.

07 August 2024 4,526 4 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Which distribution type should I use when calculating the average particle size from TEM image? and how to calculate the error ?

average particle size calculation from TEM

04 August 2024 2,921 1 View

Braj Krishna

Can you tell me the process after PMMA coating on the substrate?

Shravani Anagandula

# Braj Krishna

After PMMA coating baked it at 170 for 1 minute. then exposed to pattern developed in water:IPA (3:7) for 90 seconds. then coated gold by thermal evaporation , then substrate dipped in acetone and ultrasonicated it for less than 3 seconds.

Anil Shetti

Rines in acetone.