Shravani Anagandula

5 Questions 6 Answers 0 Followers

Questions related from Shravani Anagandula

How can i prepare a biological protien sample ?

Hello all I have a sample of protiens consisting of nanofibres of 1 to 3 nanometers diameter with long length and also comprises salts like NaCl. tried with different dilution levels, but...

27 January 2020 8,391 2 View

What is the standard lift off process in EBL ?

We have a XENOS EBL system attached to FESEM JSM-7200F After the pattern is written on PMMA we coat it with the Gold and do the lift off. Initially I ultra-sonicated the substrate in acetone just...

16 December 2019 7,784 3 View

How can I get good image of thin film of 7nm thickness on SiO2 substrate?

I have a sample containing SiO2 as a substrate ,up on that 7 nm thickness of Fe and 0.4 nm thickness of Al2O3, expected there are nanoparticles of size range of less than 100nm ,basically...

05 December 2019 3,292 7 View

Hello Dear RG members. How do I image a glass sample in FESEM ?

There are protiens and lipid samples grown on the glass .I tried pasting a carbon tape to a side and imaging but dint work out. Tried with various Acc.Voltage and probe current but didn't work...

26 November 2019 1,524 3 View

How to do 3d reconstruction from SEM images?

Hello All, I work on JSM-7200F basically dealing with thin film kind of samples and nanoparicles. so how do we 3d reconstruct from sem images? what are the softwares available for that? Is...

11 June 2019 1,257 4 View