I am trying to develop a simple method for amateurs to make relative measurements of noise in digital cameras. One method involves taking multiple images of a standard grey card, bringing the images into Photoshop as a stack and then using Photoshop's standard deviation function. As expected, the mean value of the standard deviation over the image increases with ISO setting. However, there is also a distinct spatial pattern to the standard deviation visible at all ISO settings. (See example image attached.) Changing lenses or just adjusting focus changes the pattern. No corresponding pattern is observed when calcuting mean or median.

My experiments were conducted on a copy stand using a Canon 5D Mark II DSLR and a Kodak grey card. The attached image is the pattern when the card is in focus. The contrast has been enhanced to make the pattern clearer. The standard deviation was calculate from 10 images taken in rapid sequence under close to identical conditions.

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