How credible is the average size of nanoparticles obtained using SEM image and ImageJ software?

My research work requires synthesizing nanoparticles, characterisation before application. How credible is the average size obtained from ImageJ software using images from SEM or TEM?

30 January 2021 3,838 3 View

Is it better to replicate cages/tanks or individual animals in an animal trial?

I am designing a small scale trial to test dose response to a vaccine in an animal model. The animals in question are fish, and the vaccine is administered by injection. Lets say that I have...

29 July 2020 3,784 2 View

Containment strategies for antibiotic resistance?

How can the spread of resistant genes in microbes be contained #antibioticresistance #antibiotics #microbes #bacteria

20 August 2019 3,006 4 View

Can any one share with information on or the source of ELISA for measuring Antisnake immunoglobulins in Human?

Is There a commercial ELISA Kits for measuring Antisnake venom? Please share source and cost

10 September 2018 3,342 2 View

Can anyone suggest a good protocol for precipitation and recovery of protein from P. pastoris supernatants for SDS-PAGE?

I'm trying to express secreted recombinant proteins in P. pastoris. I'm using the Pichia pink strains from Invitrogen. I've identified a few clones that express my proteins intracellularly (i.e. I...

20 December 2017 9,821 5 View

How to measure (~50 kV) very high voltage?

I need to measure high voltage in transmission electron microscope (in electron gun)

31 July 2016 520 3 View

What are the emotional, psychological and cultural impacts of ICT use on learners from marginalized and/or minority communities?

What are the emotional, psychological and cultural impacts of ICT use on learners from marginalized and/or minority communities?

12 November 2015 6,372 3 View

Does anyone know of an automatic Approach-Avoidance Task (AAT) for chocolate (or similar)?

I am conducting research into the prediction of chocolate consumption by implicit processes, and in addition to measuring implicit attitude, I want to measure automatic approach-avoidance tendency...

27 November 2013 4,270 5 View

Containment strategies for antibiotic resistance

Treatment of diseases arising from AMR has largely proved ineffective time and again. So shouldn't containment strategies be outlined to regulate spread of resistant genes?

01 January 1970 704 5 View

Does Sonication increases the defect of SWCNT?

I am working on SWCNT. After the synthesis of CNT I took Raman analysis by simply leaving material on slide. It gives GD ratio above 50 but when I sonicate it with alcohol to prepare slide, The GD...

01 January 1970 8,658 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Discrete Phase model (DPM) particle break-up has particle size limits during particle tracking?

Hello, I am currently working on simulating water droplet partciles in a closed room. When I use uniform particles, without "particle break-up", I can carry out the simulation without any problem....

02 March 2021 3,053 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View