A micro-organism might have antimicrobial activity against other organisms due to production of different metabolites, acids and enzymes etc. I want to check antimicrobial activity of some organisms focusing on their activity due to bacteriocins. Following study involves three methods to screen antagonistic activity of an organism; 1)Cross-streak method 2) Stab-Overlay method 3)Agar Well Diffusion method. I have read some other methods in other articles i.e; Agar spot method, Spot on lawn method, double plate method etc. I have understood that antimicrobial activity of bacteriocins are evaluated by testing supernatants after centrifugation cultures in broth.
Kindly guide me which method is the most suitable if someone has to check antimicrobial activity of an organism due to production of every type of antimicrobial compound,collectively by it?
Secondly, in given article, did authors evaluated this using first two methods i.e; 1)Cross-streak method 2) Stab-Overlay method?
Hello, The agar spot-test method was widely used for bacteriocin screening.
Antimicrobial activity of bacteriocins are evaluated by testing supernatants of cultures as bacteriocines are extracellular substances.
Agar Well Diffusion method is better than others for bacteriocin screening.
Sir Karen A. Darbinyan and Sir Ashwini Kumar Dubey...
i have understood from literature review that Antimicrobial activity of bacteriocins are evaluated by testing supernatants of cultures...
But i am not asking the method for bacteriocins alone... but asking which method is best if someone wants to check antimicrobial activity of an organism due to production of all types of substances by it in one method? As Bacteriocin is one compound which can have antimicrobial activity... but there are also many compounds produced by an organism having antagonistic activity...
So, which method is best to evaluate an organsim for antimicrobial activity? due to bacteriocins plus all other substances that are being produced by the test organism?
Using different methods to check for antimicrobial activity is significant because with the different methods you could strongly establish antimicrobial activity. It may help check lapses or errors in the methods used and as a result validate antimicrobial activity
I think agar well diffusion method is best to check antimicrobial activity of something.
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