The heterogeneity of biofilms increases tolerance to antimicrobials because of many barriers. In addition, the microorganisms can be resistant to the antimicrobials or they can acquire resistance determinants. I would like to know the role of biofilm formation in tolerance to antimicrobial agents, and methods that can be followed to determine it.
Biofilms are somehow a protective exopolysaccharide barrier for microorganisms against chemical and physical alterations. They also could be a serious hygienic problem in many industries especially the food ones. Some methods are well described in the literature based on coloration and epifluorescence microscopy.
Dear Sulieman,
I agree with Ahmed Zouari ’ s comment. Also, I think microplate works can do for biofilms (according to with absorbance measurements).
Best regards.
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