Dear Muthu,
there is another thread in the "question" section of Researchgate which deals with the same question. Maybe you have a look on it.
Question: "Bacillus subtilis growth condition" from Peter William Akers (University of Auckland)
Regards
This paper gives a fairly detailed procedure on B. subtilis cultivation:
Rühl M, Le Coq D, Aymerich S, Sauer U (2012) 13C-flux analysis reveals NADPH-balancing transhydrogenation cycles in stationary phase of nitrogen-starving Bacillus subtilis. J Biol Chem 287:27959–70. doi: 10.1074/jbc.M112.366492
Hi, there
To be honest, you have given little information about your question and so, it is rather difficult to answer you correctly!
I am working with bacillus subtilis for phytase production but I don't use any selective culture medium. However, if you are interested in some special aspect of this bacteria, you can easily define a medium to differentiate it from another microbes. For instance, if you are looking for a special enzyme (like me who looks for phytase), you can use the medium with phytate in it so you will observe the colonies having this trait.
in addition, there are some special media for Bacillus species important in food microbiology such as Bacillus cereous, so it would be helpful if you look at them to fine the answer to your question.
Best regards
Selective medium for growth of an organism or any metabolite production?
Glucose, Yeast extract/peptone, NaCl or tryptone, yeast extract, sodium chloride
Antibiotics: B. subtilis chloramphenicol (5 µg/ml), E. coli ampicillin (50 µg/ml) and also you can add any substitute nutrients or trace elements. the bolded elements are prefered for expression.
I think you can use the MYP agar, it´s a selective medium to Bacillus cereus but also allow the development of Bacillus subtilis.
The article referred by Michael Bradfield is no doubt a good reference to start with but see how does it meet your requirements. B. subtilis is purified for many purposes and we do not know your interest in doing so. However, in one of my recent experience I have used Nutrient Agar with Polymixin and / or Neomycin which prevented all other non sporing Gram positive bacteria. Then based on a 8 to 10 hour incubation I isolated the suspected colonies of B.subtilis using the preliminary colony characteristics as given in Bergey's Manual. This will be followed by 16S rDNA sequencing and homology studies using the most common tool - BLAST.
You may you MYP agar...Best for its growth....But need some tests for its selection from Bacillus cereus
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