What should be sample size of traning dataset for applying data mining techniques?

21 December 2020 8,119 3 View

Can I combine data from two separate fluorometeic experiments?

I performed a fluorometric experiment with two different cell lines. I had four conditions per experiment, and four "sub-conditions" per condition, wherein I add three different chemicals (the...

08 November 2020 5,113 3 View

Is it possible to calculate Absorption co-efficient or Absorbance of a dye theoretically?

I am working on a Luminescent Solar Concentrators (LSC) using dyes such as Coumarin 1, Coumarin 153, and Perylene Green Ph-probe 720-5.5. I need to calculate the Mean-free-path (MFP) for the...

18 August 2020 9,804 3 View

Is ACE inhibition linked to COVID-19?

There is increasing chatter that proteins in the ACE2 receptor binding domain are related to COVID-19 entering humans cells and subsequently infecting the host. Is there any evidence of ACE...

19 March 2020 6,635 8 View

In Phospharic Acid plant , Calendria is being used is generally of Graphite tube- shell & tube heat exchanger. Anyone using experience SS316 tubes?

Presently Majority of PA plants are using Graphite tubes in their system oh concentration for heat-exchange. Reliability is little less. We want to know that is ANY PA producing plants is having...

13 March 2020 4,877 3 View

How to model blind bolts in ABAQUS?

I am trying to simulate a beam-column connection using blind bolts. but I am not getting an idea to model blind bolts in Abaqus. please give me some idea of modelling. thanks in advance

05 February 2020 8,916 3 View

What are the possible ways to increase the internodal distance of in vitro cultures?

I am working on Papaya micropropagation where I am getting plenty of shoots but they are not increasing in height. I have tried diffuse light, GA3 but did not see improvement. Please suggest a...

03 June 2019 812 23 View

Reduction in fasting Insulin levels and associated CVD/T2D risk?

Does anyone have papers relating fasting insulin levels and associated CVD risks?

03 November 2018 4,281 3 View

What is the relationship between Stroke Volume and Diastolic BP?

Does stroke volume and diastolic BP have a positive linear correlation? Given greater pre-load and thus end-diastolic volume is it expected that diastolic BP would be reduced?

14 December 2017 9,316 4 View

Is RM-ANOVA robust enough with a non-normal dataset?

Does anyone have papers saying that repeated-measures ANOVA has sufficient robustness when analysing a non-normal dataset?

30 November 2017 7,862 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

Consensus sequence from ClustalX?

I am doing multiple sequence alignment to design primer for my gene. Can anyone tell me how to select the consensus sequence after alignment. I am using ClustalX for the alignment.

04 February 2021 8,872 2 View

What is the suitable buffer for this protein ?

I am trying to find a suitable buffer for storage of a His-tag purified protein. The amino acid sequence is pasted below. Can anyone help please? MMSFVSLLLV SILFPAIQAK QFTKCELPQV LKDMDGFGGI...

01 February 2021 5,216 4 View

Where can I find the receptor binding domain (amino acid sequence) of ALL pathogenic viruses?

I want to compare the RBD of all pathogenic viruses? Is there a database or manuscript that anyone knows of where I can access these?

25 January 2021 3,843 4 View

Luciferase assay for miRNA-gene: How to obtain DNA (PCR product) from the mutated primer?

To perform the Luciferase assay for microRNA-gene interaction its necessary a primer (wild-type) to amplificate the specific ligation site and also a mutated primer. But if your primer has a...

19 January 2021 8,568 3 View

What Contaminates Cause Loss of Resolution in Plasmid Sanger DNA sequencing?

We use an AB 3130xl Genetic Analyzer for Sanger Sequencing, and on occasion, we see our plasmid samples struggle with wavy, loss of resolution (as seen in the attached image). Our general...

14 January 2021 7,346 6 View

Why this SSR primer show band like that ?

Each time this SSR primer show band like that other show one or two band . Is it polymorphic ?

11 January 2021 2,221 5 View

Do I need to make a special plasmid for bisulfite methylation test?

I have an inducible expression of the enzyme in a newly derived bacterial strain, and methylation may be affected. I want to test methylation efficiency with a bisulfite kit. The kit is the EZ DNA...

06 January 2021 7,218 2 View

What is the best software to predict protein 3D structure when only amino acid sequence is available?

I want to predict the protein 3D structure by using computational tools. In this case, amino acid sequences are available only. What software would be best with maximum accuracy to predict this...

01 January 2021 444 3 View