What is the role of PVP in the protein extraction buffer?

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David Lyon

one use I know of is to precipitate "unwanted" polyphenolic compounds, which will "disturb" further analysis (gels and/or LC/MS).

publication:

An efficient protein preparation for proteomic analysis of developing cotton fibers by 2-DE, Yuan Yao, Yi-Wei Yang, Jin-Yuan Liu, Electrophoresis 2006, 27, 4559–4569 4559

If you should use it depends on your sample.

Nitesh S. Litoriya

PVP act as antioxidant and scavenge all reactive oxygen species which may cause degradation of protein as well as nucleic acid and denature phenolic compound.