What is the role of Phorbol 12-myristate 13-acetate (PMA) in the induction of Complement Receptor 3 (CR3) or CD11b/CD18 and CR4 or CD11c/CD18?

More Dhirendra K. Singh's questions See All

What is the percentage expression of CR3 (cd11b/cd18) receptor on Macrophages?

I am checking CR3 expression on PMA stimulated THP-1 macrophages. In my experiment i used antibodies against CR3 and checked expression by flowsight. I got nearly 20% control THP-1 cells...

01 February 2018 6,256 0 View

What is the simple and easy protocol to isolate RBC's from whole blood sample?

I have four specific questions regarding RBC's isolation from blood? Procedure-Procedure to isolate RBC's from whole blood? Can i use ficol method to isolate RBC's? my real concern for this...

31 December 2017 2,256 1 View

I want to ask about statistics used in analyzing growth curves of the microorganism?

I have two strains A and B. I grow them in a media for 0-24 hrs and took reading at every 30 mints. I plotted these values on a graph (Y=O.D and X=Time). I want to see the significance difference...

01 February 2017 8,027 3 View

Can I use Tango buffer instead of EcoR1 restriction buffer while digesting plasmid with EcoR1?

I performed restriction digestion of a plasmid using EcoR1 enzyme but I run out of EcoR1 buffer so I used Tango buffer (yellow) but I didnt get result. So I'm curious to know can I use tango...

05 June 2016 1,052 4 View

Why membrane associated complex (MAC, C5-9) of compliment system can't form pore in cell wall of Candida?

I'm interested to know why MAC complex of human complement system can't kill candida by forming the pore on cell wall candiaalbicansor other noncandida species?

03 April 2016 4,172 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

Does HEK293T cell express RIG1?

Is there any benefit in knocking out the interferon receptor in 293T cells? In the context of creating RNA viruses 📷

13 July 2024 3,382 0 View

Are there any people using biolegend anti- mouse CCR2-BV785?

Hi, I'm working on heart flow cytometry, and I use anti-mouse CCR2-BV785 (biolegend, cat150621) for staining, 20 minutes, 4 degree. But I couldn't see any CCR2+ cells. So I'm not sure if it's...

03 July 2024 3,969 4 View

What are those vacuolated structures in my tilapia intestinal cross-sections (H&E stained)? Do the sections exhibit signs of enteritis?

Could someone please confirm if those vacuolated structures are goblet cells? And whether the sections exhibit signs of inflammation. Context: I am conducting histological analysis of the...

15 June 2024 3,208 4 View

Is it hard to stain CCR2 for flow cytometry?

Hi, I'm working on the immune cells in the heart and have a problem with CCR2 marker. I did some research and found there's CCR2+ monocytes in heart failure mouse model. When I analysed CCR2...

08 June 2024 7,315 5 View

Is there anything called cd 12 in immunology?

define cd

06 June 2024 9,533 5 View

How is it possible to model a protein that consists of several subunits using a Modeller?

Good day, I am trying to model the GABA A receptor, with different subunits. I selected the templates with the highest percent of identity, but as a result of the modeling I got a protein with...

02 June 2024 5,489 2 View

How can I put an alert to receive notifications of call for papers in my research field?

I'd like to receive notifications of call for papers for any journal pertaining to my expertise (immunology, rheumatology, immuno-mediated liver involvement) from researchgate. Thanks in advance...

01 June 2024 1,352 0 View

James Drummond

I believe PMA is a diacyl glycerol analog. It is involved in similar pathways to DAG

Saleh Alkarim

Hello

i agree with James Drummond , kindly look at links here

Good Luck

https://www.google.com.sa/search?q=role+of+Phorbol+12-myristate+13-acetate+(PMA)+in+the+induction+of+Complement+Receptor+3+(CR3)+or+CD11b%2FCD18+and+CR4+or+CD11c%2FCD18&oq=role+of+Phorbol+12-myristate+13-acetate+(PMA)+in+the+induction+of+Complement+Receptor+3+(CR3)+or+CD11b%2FCD18+and+CR4+or+CD11c%2FCD18&aqs=chrome..69i57.2195j0j8&sourceid=chrome&ie=UTF-8

https://www.ncbi.nlm.nih.gov/pubmed/10529599