Dhirendra K. Singh

6 Questions 14 Answers 0 Followers

Questions related from Dhirendra K. Singh

What is the percentage expression of CR3 (cd11b/cd18) receptor on Macrophages?

I am checking CR3 expression on PMA stimulated THP-1 macrophages. In my experiment i used antibodies against CR3 and checked expression by flowsight. I got nearly 20% control THP-1 cells...

02 February 2018 6,235 0 View

What is the simple and easy protocol to isolate RBC's from whole blood sample?

I have four specific questions regarding RBC's isolation from blood? Procedure-Procedure to isolate RBC's from whole blood? Can i use ficol method to isolate RBC's? my real concern for this...

01 January 2018 2,234 1 View

What is the role of Phorbol 12-myristate 13-acetate (PMA) in the induction of Complement Receptor 3 (CR3) or CD11b/CD18 and CR4 or CD11c/CD18?

Role of PMA on the expression and function of CR3 and CR4 expressed on innate immune cells.

05 May 2017 7,264 2 View

I want to ask about statistics used in analyzing growth curves of the microorganism?

I have two strains A and B. I grow them in a media for 0-24 hrs and took reading at every 30 mints. I plotted these values on a graph (Y=O.D and X=Time). I want to see the significance difference...

02 February 2017 8,003 3 View

Can I use Tango buffer instead of EcoR1 restriction buffer while digesting plasmid with EcoR1?

I performed restriction digestion of a plasmid using EcoR1 enzyme but I run out of EcoR1 buffer so I used Tango buffer (yellow) but I didnt get result. So I'm curious to know can I use tango...

06 June 2016 1,028 4 View

Why membrane associated complex (MAC, C5-9) of compliment system can't form pore in cell wall of Candida?

I'm interested to know why MAC complex of human complement system can't kill candida by forming the pore on cell wall candiaalbicansor other noncandida species?

04 April 2016 4,154 2 View