27 August 2015 1 5K Report

What is the role of calcium and magnesium in the RPMI media in the storage of blood sample ?

More Prasanta Purohit's questions See All
In what ways does art enable individuals to express and process emotions that may be difficult to articulate verbally?

Art x Mental Health Dear fellow researchers, Art has always been a way of expression for me. It has calmed my inner turbulence and helped me to make better decisions. Let's talk about how picking...

06 February 2024 2,655 1 View

Can second harmonic generation be observed by beating of two laser beams in plasma?

When two intense laser beams interact with a plasma, is second harmonic generation produced?

23 December 2023 3,996 3 View

How to do CCSD calculation with localized molecular orbitals in PySCF?

Hello everyone, One can obtain the canonical HF orbitals and localize them by Pipek, Ruedenberg or Boys method to obtain Localized Molecular Orbitals (LMO). One can perform CCSD calculations with...

07 December 2023 3,294 0 View

From which programs an user can obtain the 2-particle density matrix from a ccsd(t) calculation?

I have been using PySCF to obtain the two-particle density matrix from a ccsd(t) calculation. However, the calculation is not so memory efficient. But, one can obtain, store ,and manipulate the...

23 October 2023 2,576 0 View

Weighing of a study in Prevalence meta-analysis?

Q 1. Which model should be preferred (FE/RE) when conducting a meta-analysis for pooling prevalence? Q2. In the RE model, why do all studies receive equal weight irrespective of sample size or...

04 October 2023 763 1 View

Can avogardo program file open in gaussian software ?

the molecule designed in avogadro program. that file can be directly open in gaussian software ?

26 September 2023 8,413 1 View

What are the signatures of a thin film on a substrate in a time domain signal obtained from THz time domain spectroscopy?

I am using Terahertz time domain system to measure the thickness of a thin film on a substrate. I am able to calculate the thickness of substrate(~100um) alone. But what signs should I see for if...

02 September 2023 5,743 7 View

GROMACS: how to calculate PMF to revolve DNA in a narrow protein channel?

I need to perform non-equilibrium simulation to push DNA through a channel such that it also revolves within the channel. The final goal is to conduct umbrella sampling simulation to calculate PMF...

16 July 2023 1,539 0 View

GROMACS: Perform WHAM with enforced rotation output file?

I performed an umbrella sampling using as CV the rotation angle of protein helix. But I didn’t use pulling md to produce configurations. I used enforced rotation to generate the configurations....

16 July 2023 6,361 0 View

Can an editor of journal upload all papers at researchgate?

Kindly let us know if a journal editor can upload all papers of a ejournal at researchgate?

10 July 2023 2,595 5 View

Similar questions and discussions
RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

If we are using snowball sampling technique, how do we justify the true representativeness of the sample statistically? is there any statistical test?

Are there any statistical methods to justify your sampling technique using SPSS or AMOS?

05 August 2024 9,153 4 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

What is the best sampling strategy?

I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...

01 August 2024 8,457 10 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Can you visualize platelets using EVOS ?

Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...

24 July 2024 9,337 3 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

I encountered a situation where the 16s PCR amplification worked perfectly with the DNA from the same sample, but using the same sample Don't working?

I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...

24 July 2024 9,688 0 View

Why my gel electrophoresis has smearing?

Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...

22 July 2024 9,761 5 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close