What is the restriction enzyme for digesting PDE4D gene SNP 45?

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How to Make a Pre-Emulsion of Methacrylic acid, Butyl Acrylate, and Acrylonitrile to use in emulsion polymerization ?

Any answer will be useful / Surfactants / Procedure I am using this pre-emulsion in sequential emulsion polymerization

14 January 2021 2,566 4 View

PRODRG server changes ligand coordinates after generating toplogy files?

I used ligand after docking and passed it to prodrg server to generate GROMOS topology files, although the files generated when analyzed were out of the binding site pocket. PRODRG changed the...

26 October 2020 1,420 3 View

I have a monoclonal antibodies (purified) of 8mg/ml, Now I want to evaluate ELISA for my serum samples in International ELISA UNITS?

I need to know how to convert micrograms of antibodies into ELISA Units.

21 September 2020 3,286 3 View

Is there any matlab code available for 28 test functions at CEC'13 Special Session on Real-Parameter Optimization?

I am currently working on PSO where i need to test my algorithm with benchmark optimization test functions. Liang et al publish this paper ( Problem Definitions and Evaluation Criteria for the CEC...

29 July 2020 5,123 2 View

Why does the transmission spectra shows an increase near the optical absorption edge?

Measuring a transmission spectrum of thin film (eg: single phase oxide) using UV-Vis spectroscopy, the transmission value gradually increases as we decrease the wavelength and reaches a peak until...

30 April 2020 700 1 View

Do you have GC-MS datasets that are not analyzed/ published yet?

Hello, I am looking for unanalyzed and unpublished GC-MS datasets from diverse mass spectrometer vendors ? Such as ToFs, single/ triple Quads, Orbitraps etc. that have been acquired from human,...

10 April 2020 2,347 10 View

Is Gravitational Force the result of spread of Energy?

Mass attracts and Energy spreads, Both Matter and Energy is essentially one and the same thing interchangeably but still it differs in this respect that Energy spreads, Sir Isaac Newton in his...

08 February 2020 5,016 3 View

How much primer designing differs from probe designing for exome sequencing? What if we design biotynylated primer instead of probe for capturing?

What are the criteria for designing biotinylated probes differ from primer designing?

24 September 2019 8,205 2 View

I Need aspen help in coal simulation ?

i tried to do aspen simulation of coal gasification but am not able to do so, i have been trying for past 2 months !! could any one help me by making me a simulation file so that i can understand...

04 September 2019 7,420 3 View

Hello, I need solar irradiance data, Uttar Pradesh, (hourly, any day in May) for any city (if mahoba, jhansi is there then blessing in disguise)?

Working on impact of higher penetration of solar generation on Thermal Generation: Case Study for Uttar Pradesh, India. If any one has hourly irradiance data please share.

01 July 2019 5,610 9 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View