I am doing RFLP of the PCR products of PDE4D gene SNP 45. Thus, I need to know the restriction enzyme for its digestion.
if you included the sequences in the question Shubham it would get you a quick answer to tis question
it appears to be hpych4iii please check the attached sequence and restriction site from neb cutter
paul
Thank you so much. Your help is highly appreciated.
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