What is the relationship of presence of interleukin (IL) in the serum with the presence of pathogenic bacteria in the body with example?

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Please, what is the scientific name for this plant?

This plant grows in my garden.

26 February 2017 7,276 3 View

This plant is used in the treatment of diabetes, but I do not know the scientific name of the plant. Please, what is the name of this plant?

The seeds of this plant brought from India.

28 September 2016 9,673 20 View

How can I block infrared light through the force of polyethylene?

Are there pigments can be painted by polyethylene membrane working on the absorption of infrared light?

07 December 2015 1,357 3 View

Research and Truth

What factors influence the degree of telling the truth in academic research? I have humanities in mind, particularly politics related topics. How much where a researcher comes from influences...

01 January 1970 6,447 16 View

Research between Innovation and Imitation

I wrote an article that I sent to be considered for publication for several journals. One prestigious journal rejected the article on the basis that it does not contribute to the common ground of...

01 January 1970 5,238 22 View

Word limit in academic research

How do you feel about setting word limits as a requirement in publication or even in post graduate thesis or dissertation writing?Are you comfortable with it? I realize that there are technical...

01 January 1970 4,111 17 View

What field reconciles video games, marketing, and communication technologies?

My son is fond of video games. He is also fascinated with digital marketing, computers and mobiles. He would like to hear suggestions on what academic field(s) or specialty fit his interests so...

01 January 1970 6,291 10 View

Lying, the distortion of truth, and pretense in literature

Lying, the distortion of truth, and pretense...... I wonder if you guys could suggests any poetic works in which these themes appear. I'm investigating the subject from a literary view point with...

01 January 1970 6,173 29 View

What aspects do university writing centers usually cover?

Trying to investigate how students' academic skills can be improved in a way that enables a better integration with university study and research requirements. What aspects do university writing...

01 January 1970 5,514 2 View

How far does the rejection of stereotypes or widely circulated statements divert us from seeing the truth?

In mainstream intellectual humanitarian discourse we are often encouraged not to stereotype. While stereotypes are not always right, they tell part of the truth. So how far does the rejection of...

01 January 1970 472 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

How to prevent hemolysis after performing cardiac puncture to draw blood from mice in serum?

We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?

29 July 2024 8,402 4 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

Does anyone notice the tumor regression impact of Hepa1-6 cells in HCC orthotopic mice model?

To establish an orthotopic HCC model, C57BL/6 mice (male, 6-8 weeks) were orthotopically implanted with 3x106 Hepa1-6 cells (Over-expressed with a human gene) in 20uL Matrigel into the left lobe...

18 July 2024 9,177 2 View

ELISA sandwich with two monoclonal antibodies ?

hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...

16 July 2024 9,044 10 View

Differentiation THP1 cells into M2 macrophage with IL4 and IL13?

Recently, I tried to differentiate THP1 into M2 macrophage using IL4 and IL13 (purchased from R&D). This is my protocol. THP1 was seeded into 6-well plate (10E6 cells/well). Incubate with PMA...

15 July 2024 5,153 2 View

Why is the baseline fibrosis level of HK-2 cells very high?

In many experiments where HK-2 cells were stimulated with TGF-β at 10 ng/mL for 48 hours, I observed that even in the control group, the baseline expression level of fibrosis proteins like FN1 and...

15 July 2024 1,929 2 View

What do I do with dried urine samples?

I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...

14 July 2024 6,724 0 View

The HCP removal rate is lower than usual in concentration step, why?

Hello everyone. I am working on my LV concentration porject, I use 500Kda hollowfiber to do it. The HCP and BSA removal rate is nearly 80%, but last time, I failed to run it. The HCP hasn't been...

11 July 2024 5,380 0 View