How to calculate MDA content from absorbance values at 532 and 600 nm?

in analysing MDA content in plant tissues, I took 0.2 g leaf tissue (fresh welght), added 5 ml 0.1% TCA and samples were grind. samples then were centrifuged at 12500 rpm for 20 minutes. 3 ml from...

16 July 2019 6,884 2 View

How can I store stool specimen for Molecular diagnosis for human parasites Is there a difference of storing for PCR or real PCR or multiplex ?

Molecular stool

08 January 2019 8,186 3 View

How you calculate the sample size for project on a clinical disease?

How you calculate the sample size for project on a clinical disease?

21 July 2018 9,389 9 View

Can somebody tell me the most common microorganism isolated from ICU?

Can somebody tell me the most common microorganism isolated from ICU?

23 July 2017 9,542 5 View

Why some planes in orthorhombic structure have four miller indices?

we know the orthorhombic structure has three axes and hexagonal structure has four axes. therefore, the miller indices for orthorhombic structure and hexagonal structure are (hki) ,(hkil)...

13 February 2017 5,956 3 View

No title

07 January 2017 7,604 2 View

Why we must modify pH value in sol-gel method ?

23 May 2016 906 9 View

Does anyone know some papers that studied thermal analysis (TGA-DTA) and (FTIR) for CaBi2Nb2O9 ceramic?

I want to these paper please .

16 October 2015 9,480 2 View

How do I test normality for two independent groups?

Hi, I am doing comparison between two independent groups; patient group of 24 subjects and control group of 34 subjects. My questions are: 1) Is testing normality relative? I mean is it sufficient...

07 October 2015 5,646 21 View

How can I correct results for a substance measured in two different machines?

10 June 2015 927 6 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Any easy method of analyzing vitamin D in foods by HPLC?

Looking for method of extracting and analyzing vitamin D in foods by HPLC

24 February 2021 7,524 7 View

Is it possible that Cancer cells could survive in heparinized blood for 48 hours?

Hi everyone, I am just wondering if we incubated 200 microliter of whole blood with 50 microliter of cancer cells(1bout 5000cells) do you think that cancer would survive for 48 hours?

23 February 2021 3,840 3 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Can the viability assays (ex: MTT/CCK-8) measure cell proliferation? or are they solely viability assays?

I want to conduct a proliferation assay to test a chemotherapy combination on a cancer cell line. At first, I thought about the MTT assay. But now, all sources are saying that these do not measure...

14 February 2021 3,142 7 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View