Which fluorescent filter is required for observing GFP encoded protein in bacterial cells ?

GFP is encoded in bacterial cell, which filter is required to observe such cells under a fluorescence microscope?

21 January 2020 2,561 3 View

Can we analyse the morphology of liposomes by Scanning Electron Microscopy (SEM)?

Can we analyse the morphology of liposomes by Scanning Electron Microscopy (SEM)?

19 July 2019 9,874 4 View

How to do in vitro enzymatic hydrolysis study of lipophilic prodrugs?

We have to carry out in vitro hydrolysis of lipophilic prodrug (log P 3.39) to drug in the presence of enzymes. As the drug is having very poor solubility in aqueous phase (phosphate buffer pH...

26 September 2018 6,971 2 View

How to clone the 3' UTR of gene whose length is very small?

3' UTR of my gene of interests spans in a total of around 120 bp, although the 5' UTR is huge in kbs. I am not able to design suitable primers to clone the whole UTR for luc-assay. Tried making...

23 July 2018 6,889 4 View

How to "equalize" these two data plots?

I have two data from two different sensors (Blue = Sensor 1 and Red = Sensor 2) 1.png . Sensor 2 is the gold standard and I wish to recalibrate sensor 1 so as to get an "equalised" data plot like...

19 June 2016 2,750 3 View

What are the sources /causes of channel estimation error (CEE) ?

06 April 2016 1,772 2 View

My cell line U87MG is getting detached after splitting, what could be the reason?

Few weeks back there was fungal infection in some of my petri dishes. So, I thoroughly cleaned the incubator using bleach and rectified alcohol, revived fresh stocks, prepared everything fresh and...

24 August 2015 9,535 16 View

How can I hybridize the Error Back Propogation algorithm with the Genetic algorithm in Matlab?

I am using this method to predict the stock market.

16 March 2015 8,098 2 View

Can we perform flow cytometry on -80 frozen samples?

We collected the plants 2 months back and stored them in -80

26 April 2014 8,841 4 View

Under which filter should I view acridine orange and Etbr staining for checking cell viability of cancer cells?

I just want to know the cells should be viewed under which filter.

15 April 2014 1,549 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How can I set up a good fit SEM model in AMOS?

I am trying to make an SEM model in AMOS for my dissertation but having some trouble with getting a good fit. I am looking for relationships between self-efficacy reported on a Likert scale and...

26 February 2021 8,642 4 View

How could I process data in RCT to include it in meta-analysis?

I'm in the process of doing a meta-analysis and have encountered some problems with the RCT data. One of my outcom is muscle strength. In one study, I have three different measurements of muscle...

25 February 2021 7,603 3 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

Am i able to conduct a factor analysis on each of my subscales, as opposed to the whole scale?

My psychometric scale contains 38 items in total, with 4 subscales (20, 6, 6 & 6 items respectively? Can i run a factor analysis for each subscale rather than the scale as a whole for the...

24 February 2021 8,856 8 View