What is the ranges of total Supper Oxide Dismutase (SOD) in Human Serum?

More Kaniaw Rafhat's questions See All

How long can enzymes be frozen without being degraded?

Like Superoxid dismutase For ELISA technique

15 May 2017 7,058 3 View

Does anyone have a method to mesure skeletal muscle decline and neuromascular disorders in old adults?

to find any molecular changes in old adults and to known reason of shaking in old adults.

17 June 2016 2,887 4 View

How can I calculate the SOD enzymatic activities using the spectrophotometer method?

I have to quantify the enzymatic activities of SOD in plants using a method that employs hypoxanthine-xanthine oxidase to create O2. The colorimetric reaction was performed by combining...

23 July 2024 8,492 3 View

Why SOD of samples are higher than control?

Hello I'm measuring SOD activity in plant leaves using NBT method. Reaction solution contains EDTA, riboflavin, methionine and PBS. But my samples values are higher than control. Do you know how I...

09 May 2024 8,930 1 View

What should I use to homogenize samples for measurements of Oxidative Stress?

I bought a lipid peroxidation kit from Sigma and I want to get kits to measure nitrates, superoxide dismutase, catalase, and glutathione. Each one of those kits comes with a different lysis...

07 May 2024 4,583 2 View

Kit for SOD assay in Plants?

Can anyone suggest any kit for SOD activity in plant? I have the protocol for NBT reduction but don't have centrifuge for falcon as the protocol requires 3mL of homogenate to be centrifuged in...

06 May 2024 1,411 4 View

Incubation of antioxidants with viral inoculum experiment?

I would like to understand whether a specific frequency of light (in the visible spectrum) inactivates a viral species by oxidative process through ROS formation or by a conformational change of...

05 May 2024 2,109 0 View

Should I use the supernatant or lysate part of the cell?

When evaluating the expression of superoxide dismutase, catalase and glutathione peroxidase enzymes by ELISA in cell culture studies, should I use the supernatant or lysate part of the...

24 March 2024 5,247 2 View

What is the appropriate conversion factor to convert the units of measurement for antioxidants from Micromol/L to international Unit?

A For dear researchers, we have a group of variables that were obtained in different units, which are the enzyme superoxide dismutase , Glutathione and Malondialdyhyde in micromol/liter and...

18 March 2024 5,293 3 View

Can any one tell me the indirect method to calculate SOD based on Quercetin?

Full method and formula to calculate SOD based on Quercetin in animal tissue

08 March 2024 6,303 0 View

Creating oxidative stress model in cell culture?

Hello everyone! MTT was performed after applying DEHP to HeLa cells. According to MTT results (24 hours of exposure), cell viability is significantly reduced at concentrations of 100 micromolar...

27 February 2024 503 2 View

Can I use triphenyl tetrazolium chloride (TTC) instead of nitroblue tetrazolium (NBT) to estimate SOD activity?

Both TTC and NBT yield formazan when interacting with oxides. The superoxide dismutase assay requires NBT, which reacts with oxide to form formazan. TTC also produces Formazan when it interacts...

12 February 2024 5,006 1 View

Amel Amrani

3.70 ± 1.53 U/mL

http://www.biomedres.info/biomedical-research/serum-total-superoxide-dismutase-enzyme-activity-in-type-2-diabetic-patients-with-retinopathy-in-mthatha-region-of-the-eastern-cap.html

Kaniaw Rafhat

Thanks Mr Amel

Jabbar A A Al-Saaidi

2-5.5 U/ml. Also it usually increased with progress of age.

can you give any paper about increasing of SOD with progress of ageing?