How do Standard compounds help in TLC with crude extracts? Specifically when we look for flavonois we use flavonoid standard.
It wil not help in the crude extract , unless if they are present in large percentage , i do prefer doing fractionation of your extrcat then check the starndard , you can even do H NMR for the factions and comapare with the standard. If you have LCMS this is the best.
Agree with Mohamed, at low contentration it will not help. TLC is great, quick easy cheap.... but it has a lot of limitations, one being: what you see is NOT always what you get.
I should say that the intended purpose is to just confirm the presence of standard compound in your test extract. If you intend to isolate the standard from the test sample, then the reference compound is useful for identification. At every step during your isolation process, you have a reference to match with and you can track the isolate in the fractions isolated. That's it. You will have to reconfirm its presence using HPTLC, HPLC, FTIR, MS or NMR for proper characterization and structural elucidation.
That's why TLC is considered as a qualitative method for chromatography. There is every possibility that during HPTLC you get a different profile with more spots than what you got from TLC in the same solvent system because HPTLC is more advanced and versatile.
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