What is the purpose of adding DTT to Laemmli buffer when loading samples into a western blot?

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Microwaving food: good enough for humans but not cells in culture?

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In immunofluorescence staining, will the presence of phosphate in buffers affect the phosphorylation status of proteins of interest?

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Is it possible to over-stain with DAPI?

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Should I use a protease/phosphatase inhibitor when conducting immunofluorescence?

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How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

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How optimize the Size Exclusion Chromatography?

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Why are my bands weird in this Agarose gel?

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Will a 10xHis-tag prevent anti-MBP from binding to MBP?

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Adam B Shapiro

If disulfide bonds are not reduced, the residual structure of the protein may cause it to run at an incorrect molecular weight.

Can Kiessling

To answer this question, you should also understand the differene between native and SDS-PAGE.

https://www.researchgate.net/post/Can_anyone_detail_the_differences_between_Native_PAGE_and_SDS-PAGE