What is the purpose of adding DTT to Laemmli buffer when loading samples into a western blot?

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Adam B Shapiro

If disulfide bonds are not reduced, the residual structure of the protein may cause it to run at an incorrect molecular weight.

Travis I Moore

DTT is a redox reagent that reduces disulfides to their corresponding thiols.

Can Kiessling

To answer this question, you should also understand the differene between native and SDS-PAGE.

https://www.researchgate.net/post/Can_anyone_detail_the_differences_between_Native_PAGE_and_SDS-PAGE