What is the protocol to neutralise/ desactivate Na-azide ?

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A. M. Naim O'Sullivan

Hi Clemence, this protocol might help but I haven't tried it myself yet.

http://www.ehs.neu.edu/hazardous_waste/fact_sheets/sodium_azide/

Clemence Bonnot

Thank you ! I will see with our security officer how to set it up but if people have simpler options I would have a look at it too!

Viviana Spotorno

Unreacted NO2- exces is eliminated by the aditionof amonium sulfamate in water (5mg/ml) until thereis no more buble, (aprox: (2 Vreaction/1 Vsulfamate)). After conjugation to protein, the conjugate is purified by centrigugal filter device (Centricom), which provide concentration and desalting of macromolecules.