I performed Red Oil staining to stain lipids. I had a lot of background at the beginning with red oil drops "on top" of the plan of the cells. I changed a few steps to remove as much background staining as possible but I am not sure if my staining is properly done and if I can use it to analyse important tissues. Could you give me your opinion about the staining and tell me if you think it is ready to analyse ?
Many thanks !!
Hi Clémence,
Quite big lipid drops but your staining seems very good.
I think it is ready for analysis.
Is that steatotic liver?
Thank you Patrick Rouimi, this was steatotic liver from an obese mouse as a positive control. I performed a negative control by dipping slides into acetone and all the staining was removed without background (so it was specific staining). I then performed the "real" stainings on my important slides and got very nice results ! Thank you very much for your feedback.
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