You should visit NCBI.

No

Well, it depends upon how they are located in the gene map, if they are very close then it's difficult but possible if they are 10-20kb apart from each other. Otherwise you might get a band of 2 genes together. If they are 10-20kb apart then you can just mix 3 primers F+R to make a primer master mix and put 6ul each reaction of the colony. Choose a close by annealing temp for all 3 primers should work. For example, primer 1-56C, primer2-62 and primer 3- 57. So, I would set up Tm at 57C. Again it will be less complicated and crisp if you set up 3 different reaction for different genes.