What is the proportion of mesenchymal stem cells within the bone marrow of mice?

04 February 2015 1 8K Report

I have some problems with finding out what the proportion of mesenchymal stem cells is that you can find within the bone marrow of mice. Does anyone know anything about it and can quote some literature? Thanks a lot.

Mohammad Gh. Zadeh

Hi,

It has reported that the ratio of MSCs in the murine bone marrow would be about 1 per 11,300-27,000 nucleated cells. You can see the following paper for more details:

- Meirelles & Nardi., ‎2003, Murine marrow-derived mesenchymal stem cell: isolation, ...

Badges
Science topic

More Anja Osterberg's questions See All

How to do advanced search?

I need advanced search like on Pubmed

30 May 2024 4,984 1 View

How do I create a APA reference?

how do I create/copy a APA reference automatically from an article

21 January 2024 2,968 1 View

What is the effect of Safewards on numbers of aggression in psychiatric care?

Dear researchers, I'm a master student in Belgium and I also work in psychiatric care for more then 12 years. I would like to recieve some tips or specific articles on exact numbers of...

16 March 2023 3,442 4 View

What is the impact of national culture on family firms?

Dear all, I would like you to recommend me some papers that explore the impact of national culture on family firms, as it is a topic I would like to explore on my thesis. Thank you for your help.

02 March 2023 8,640 3 View

How to determine cutoff/threshold for rt PCR for Cutibacterium acnes?

Hello. I have a problem with our in-house designed rt PCR for Cutibacterium acnes. Primers and probes were designed as part of mPCR. While testing each set of primers (monoplex) for LOD we are...

01 March 2023 4,356 1 View

How to transfect H292 for CRISPR k.o.?

Dear all, I am want to generate a k.o. with CRISPR/CAS9 in H292 cells and have issues with the transfections. It does not matte if its for plasmid or RNP format. Does anyone have a working...

02 September 2021 1,360 2 View

How to storage samples with acetonitrile without loss?

Dear all, we preparad samples with acetonitrile, nevertheless we have to storage them for some time. How can we avoid loss of acetonitrile in these samples? We tried storaging at -80°C,...

22 July 2021 5,273 2 View

Can I use asymmetric PCR amplification on a pool of circular plasmids to increase each plasmid's signal?

Hello, Here is my experiment: I transfect in a plasmid that has been cut once (a linear piece of dsDNA) into human cultured cells. I wait 48hrs to allow the cells to "repair" the plasmid making...

10 February 2021 6,403 3 View

What is the red autofluorescence in my HL60 cells?

A subset of my HL60 cells has red autofluorescence in the far red region (FL-3 > 670 nm). I first noticed this some months ago when I was checking an antibody with a PE-Cy5 tag by flow...

06 December 2020 6,189 2 View

Best ways to collect saliva from guinea pigs?

Hello, I am planning a reserch which involves analysis of saliva cortisol in guinea pigs. Unfortunately, I am currently having trouble collecting saliva samples from my guinea pigs. I am not able...

30 September 2020 9,407 1 View

What are the emerging trends and opportunities for STEM education and careers in the online space?

10 August 2024 507 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

05 August 2024 10,079 0 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View