What is the red autofluorescence in my HL60 cells?

A subset of my HL60 cells has red autofluorescence in the far red region (FL-3 > 670 nm). I first noticed this some months ago when I was checking an antibody with a PE-Cy5 tag by flow...

06 December 2020 6,005 2 View

Best ways to collect saliva from guinea pigs?

Hello, I am planning a reserch which involves analysis of saliva cortisol in guinea pigs. Unfortunately, I am currently having trouble collecting saliva samples from my guinea pigs. I am not able...

30 September 2020 9,226 1 View

Transfection in HFL-1 cells?

I am trying to transfect HFL-1 cells with a plasmid DNA. I used Lipofectamine 3000 from ThermoScientific, however the cells seem to react quite sensitive to the transfection (tried different...

17 November 2019 484 2 View

Is there a full list of scales from the FOCUS Questionnaire (van Muijen et al., 1999)?

Hi! I'm currently working on my Master's thesis and looking for the evaluative scales for the innovation and rules value orientation from the FOCUS Questionnaire developed by van Muijen et al....

05 May 2019 4,039 3 View

I have putted the 10fold amount of 2-Mercaptoethanol in my stripping buffer by mistake. Does anyone have experience with such a mistake?

I think my proteins on the PVDF membrane are gone, because there is nothing to detect anymore. But on the other hand the membranes smell after a lot of washing procedures still of 2-ME. Maybe my...

18 July 2018 171 3 View

Is there any relevance theoretical work that addresses the question of folk psychology?

This question is in connection with an article I am writing. Relevance theory seems potentially helpful for my argument, but I need to find work that explicitly addresses the link between the...

28 September 2015 8,493 3 View

Can anyone provide advice on how to model age captured in categories within SEM?

10 July 2015 4,972 15 View

How can I regenerate a Pt surface after polymerization?

I have a Pt crystal I would like to reuse. The Pt surface has been electrochemically grafted with a diazonium salt and subsequently polymerized with GMA. I'm thinking it should be possible to...

09 July 2015 5,149 2 View

Is there any recent work on literary language (or the nature thereof) or of "literariness" more generally?

Critics don't seem to be interested anymore in distinguishing between ordinary and literary language, but I'm wondering whether this is a crucial issue we need to return to.

06 October 2014 8,200 5 View

Can anyone suggest a material with the highest specific heat capacity (higher than water)?

Hi, I was wondering which material has the highest specific heat capacity? Most lists I could find have water at the top with 4.182 kJ/kgK. But I was wondering if there is a solid or liquid...

17 September 2014 5,850 3 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Failed Chromatograms on HPLC-ESI Orbitrap?

Hello fellow researchers, I recently have a problem with an HPLC coupled to an Orbitrap mass analyzer. I usually use some protein mix to make sure the equipment is ready to separate and read...

09 February 2021 3,595 8 View

How can I analyze using NMR spectroscopy; my compound is only soluble in hot methanol?

Halo every one I have isolated a compound from the methanolic plant extract. The compound is UV positive at both 254 and 365 nm. It seems to be insoluble in most solvents like CDCl3, CD2Cl2,...

13 January 2021 2,267 3 View

Agmatin determination with LC MSMS?

I would like to determine agmatine (C5H14N4 CAS 306-60-5) with LCMSMS. The samples are sport supplements. The processing should be just dilute and shoot . I have not yet succeeded in achieving a...

29 November 2020 8,356 4 View

What should be the ideal solvent for meso-tetra(2,3,4,5,6-pentafluorophenyl)porphyrin?

I've tried using Acetonitrile but the solubility is very low.If there's any cited articles that'd be very helpful!

22 November 2020 6,929 3 View

How can I dissolve biphenyl in carbon-free minimal salt media effectively?

I prepared a 5X of M9 minimal salt and 1000X elemental trace and mixed these 2 solutions to produce carbon-free minimal salt media. Biphenyl was used as a carbon source but was prepped separately...

20 November 2020 5,904 5 View

HPLC data show negative peak on the blanks, please can I solve the problem?

09 November 2020 5,884 5 View

Can i make sample solution (stock and working ) in methanol and analyze sample with hplc mobile phase acetonitrile and water ?

i am confused if sample should be prepared in mobile phase as used in HPLC Will the different solvent for sample and mobile phase being different affect peaks or any component of anlysis ?

03 November 2020 1,656 2 View

What would cause a bathochromic shift (lambda max increase) of protein?

I purified a small protein using HPLC on an acetonitrile (+0.1% TFA) gradient. I then lyophilized the HPLC fraction and resuspended the freeze-dried sample in sodium acetate, potassium nitrate,...

20 October 2020 2,833 1 View

Is Tetrabutylammonium hydroxide Mass Spectrometry compatible?

Hello forum, I would like to know if TBA.OH is volatile in mixtures of acetonitrile or methanol. I want to analyze peptides that were previously dissolved in TBA.OH-conatining solution, but dried...

05 October 2020 6,936 3 View