In the spleen, T-lymphocytes will be responsive to IL2. IL2 is required for T-lymphocyte development in the thymus, and then is critical in mounting an immune response as IL2 stimulates the IL2 receptor to cause proliferation and activation of T-lymphocytes. The response will be primarily CD4+ and CD8+ T-lymphocytes. Hope this is helpful.

~Adam

Thanks!

Fritz

Naive T cells express low affinity IL-2R - which respond poorly to physiological levels of IL-2. However, T regs constitutively express high affinity IL-2R and will be responsible for most of the proliferation - detected as "background" levels in naive spleen. Antigen-specific, activated T cels express the high affinity IL-2R. This is the basis for utilizing the IL-2 proliferation assay to assess immunization protocols.  In general - there should be a dose response of antigen dose, or boosts, to the magnitude of cell proliferation.   But optimal proliferation will require the addition of antigen, antigen presenting cells, in addition to IL-2

IL-2 will stimulate directly or indirectly almost all spleen cells: T-, B-cells, Mo/Mf, DC. By direct: all cells with IL-2 receptors; indirect by stimulating T-cells to secrete other cytokines which will stimulate other cells.  Of course, it will depends on the concentration and time of exposure to IL-2 and ratio of the cells in the culture (e.g. monocyte-depleted or not).

Thank you very much for your comments!  They are very much appreciated.

Fritz