Greetings,
I have culture filtrate and cells of bacteria and I'm going to do catalase and peroxidase activities for them (Antioxidant activities).
Can any body tell me what is the reagents used in the assays.
I also want what is the procedure and method for catalase and peroxidase activities.
Thank you so much
Hello Nasir Ali Khan!
You can follow the Abei et al. (1974) method for estimation of catalase level in your cell/tissue sample. DOI: 10.1016/b978-0-12-091302-2.50032-3
Please let me know if you face any further difficulty in this one. I have experience on measuring CAT level in brain tissue, but I haven't perform any analysis on POD activities.
Hope this comes in handy to you.
Guaicol peroxidase (GPOX, EC 1.11.1.7) activity was measured in a reaction medium composed of KH2PO4 / K2HPO4 (50 mM); pH=7; H2O2 (10 mM) and Guaiacol (9 mM) by monitoring the increase in OD at 470 nm due to the formation of tetra guaiacol (Fielding and Hall 1978).
Catalase (CAT, EC 1.11.1.6) catalyzed the degradation of hydrogen peroxide (H2O2). The activity was measured in a Phosphate Buffer (50 mM; pH= 7); H2O2 (10 mM) and DTT (1 mM) by monitoring the decrease in OD at 250 nm (Aébi 1984).
The simplest method for catalase is a method by Aebi it;s simple and easy .
Chapter Catalase
there are many recently developed methods Article Simple spectrophotometric assay for measuring catalase activ...
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