What is the probability of getting chlorophyll mutants in invitro shoot tip culture of banana if it is subjected to gamma irradiation?
I wish to resolve IL-6 (mol wt= 21-26KDa), TNF- alpha (17.3-17.4 KDa), GMF-beta (16.7 KDa), GAPDH (36kDa) and PDL1 (40-70 KDa). I think 12.5% gel is good to go. Is it advisable or should I go for...
25 April 2024 9,798 0 View
I am running ELISA for different biomarkers against meningitis. I need to know how to calculate cutoff values for it in ELISA. There is no negative control or positive control for these kits,...
07 April 2024 3,079 1 View
I have selected two deep learning models CNN and sae for data analysis of a 1 d digitized data set. I need to justify choice of these two dl models in comparison to other dl and standard ml...
18 January 2024 6,800 1 View
I have used two dl models CNN and sae for data analysis of 1 d digitized data set. I need to justify use of these two models in comparison to other dl and other models. I am using ga to optimize...
18 January 2024 9,816 0 View
I am working on 16s rRNA gene sequencing. So for that I should do PCR with random primers. Suggest me with a primer that I should use, also the which universal buffer the ti can use and if...
14 December 2023 6,288 0 View
Is it usual to use the closed ended questions in a Google sheet before carrying out semi-structured interviews for collecting data in a phenomenological study? If it is possible how can it be...
08 December 2023 2,290 7 View
For the measurement of particle size from XRD TEM SEM etc
12 November 2023 8,821 2 View
Currently, I am working on mouse or rat models' primary culture of liver cells (particularly hepatocytes). In most of the previous research, I found the protocol stressing the perfusion of the...
10 October 2023 3,685 0 View
Required to understand how to solve potential flow around a ship hull.
29 September 2023 6,949 0 View
I know that many papers get 0 citations. I have read that 1.8% of papers get more than 100 citations. How about 200? What percentage of papers get 200 or 500 citations?
10 September 2023 5,973 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View
Hai, Iam doing experiment about novel compound to induce thermogenesis on 3t3-L1 cells. The beta actin has been adjusted, but when i did western blot and imaging with image J, the result it is...
14 July 2024 3,354 1 View
Also, explain its pros and cons concerning other organic sources of nutrients.
01 July 2024 612 0 View
Hi everyone, I am currently trouble shooting my restriction digestion, so far it hasnt given me clear bands when I ran gel. I've been adding all reagents minus template, to make a master mix...
24 June 2024 1,171 3 View
We coat the plate in poly-D-lysine (50 ug/mL) followed by fibronectin (50 ug/mL) before plating the cardiomyocytes. After isolating and plating cardiomyocytes from 2-4 day old pups, we fed the...
20 June 2024 393 0 View
can any one provide shooting method in matlab for 9th order differential equations with 9 boundry condition(Velocity, Temperature and concentration)
14 June 2024 7,928 2 View
test on particle board by density, water swerlling and tensile strength. Just need example amount how much we use in this experiment
29 May 2024 5,366 0 View
AAZ
11 May 2024 7,466 2 View
I searched in literatures and i got the formula root length of treated plant / original root length *100 when i applied this, i got the values higher than 100.
10 May 2024 9,148 2 View
I am reaching out regarding the issue of conversion devices/auto sears/switches that make it possible to convert semi automatic pistols and rifles into full automatic. These devices are...
19 April 2024 8,796 0 View