recently i have some researches about histopathological changes in the bone , therefore i want to know the typical properation methods to (SEM ) photos . .
Depends on what are you looking for in a bone. Bone could be mineralized or demineralized, fractured or embedded. I bit more information is needed.
Article Scanning Electron Microscopy of Bone
https://onlinelibrary.wiley.com/doi/abs/10.1002/(SICI)1097-0029(19960201)33:2%3C92::AID-JEMT2%3E3.0.CO;2-0
https://www.sciencedirect.com/topics/social-sciences/scanning-electron-microscopy
https://qmro.qmul.ac.uk/xmlui/bitstream/handle/123456789/57295/Chapter%2034%20-%20Boyde%20-%20Text%20-%20V1%20accepted%20Jan%202019.docx?sequence=2&isAllowed=y
I am very thankful for your interest .
It depends on intended purpose and bone type..
https://link.springer.com/chapter/10.1007/978-1-59259-417-7_34
Chapter Sample Preparations for Scanning Electron Microscopy – Life Sciences
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