How to isolate DNA nanoflowers after rolling ring amplification
I am working with a material which is sensible to water and oxygen, I would like to oxidize this powder either chemical or electromagnetically. Does anyone knows a method to electrochemically...
09 February 2021 2,944 3 View
In COMSOL Application Library, there is case about waveguide adapter. But there are some questions: 1) In boundary mode analysis, it just set to find only 1 mode and the S-parameter is...
12 January 2021 9,383 2 View
Hi, I am trying to generate a structure file through PGDSpider, for a diploid organism, where each locus is in two consecutive columns in one row, rather than data for each individual be stored...
29 December 2020 5,169 1 View
In some experiments for investigate drug effects for in vivo xenograft model, some authors onle give treatment to the mice for 14 days or 21 days, but the data collection/observation (such as...
13 December 2020 525 2 View
The current distributed reinforcement learning framework, such as IMPALA, uses the structure of centralized learning and distributed execution. I feel that this is not really distributed. Is there...
02 December 2020 5,598 4 View
Now, I have got a metal-doped perovskite in which blue shift happens result. I tried to make it a more blue shift to get a stable and very blue perovskite. So I think is there any possible...
04 November 2020 8,384 3 View
I doped Mn into perovskite. I found when up to the best doping amount, the thin film PLQY much higher than the dispersion PLQY in my experiment. I don't know what caused it, is there any theory...
04 November 2020 2,477 3 View
Hi, I'm a graduated Ph.D. trying to land a system engineer/instrumentation engineer position at an X-ray vendor company such as Rigaku and I have some questions about this: 1. How to standardize...
25 October 2020 7,673 4 View
I added the Mn dopant into the MAPbBr3 perovskite. When Mn dopant I added up to a certain amount, pl intensity can get a high result, but in the next day, the highest result I got decreased...
05 October 2020 9,192 7 View
How do I change insoluble recombinant proteins to soluble proteins? My recombinant protein is Aldose dehydrogenase from bacterial with around 35.0 kDa size. Now, My expression vector and...
05 October 2020 1,840 2 View
I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance
03 March 2021 3,568 1 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...
01 March 2021 3,355 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View
Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
25 February 2021 6,969 3 View
I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...
24 February 2021 4,397 3 View
23 February 2021 4,014 3 View
I got a thin band and a thick band i guess it would be the genomic DNA and the 260/280 ratio is 3.
21 February 2021 6,523 6 View
Hello, I hope you have a good time. I work on a research project about temperature indices. Due to the high number of indices, I only work on tables and maps on an annual time scale. In other...
19 February 2021 9,742 2 View