What is the optimum signal to inject the sample in Ion exchange chromatography (IC)?

Anandharaj Marimuthu @Anandharaj-Marimuthu

31 December 2013 1 9K Report

The total signal is always varying from 10-3, so I confused which signal is the correct one for the injection. Anybody can help me?

Vincent Voorwerk

Hi Marimuthu,

Are you still having trouble with this? If so, what do you mean by total signal?

The response (for example μS)? Usually the baseline will become stable as the IC will settle after 20-30 minutes.

Regards, Vincent

Badges
Science topic

Similar topics
Chemistry
Organic Chemistry

More Anandharaj Marimuthu's questions See All

How to design guideRNAs that should target all the alleles of a gene in monocot?

In banana, we are having multiple alleles. For distrusting the function of a gene through CRISPR/Cas9 it is important to mutate all the alleles through designing specific guideRNA repeats. How to...

15 August 2022 5,313 1 View

How to do Fluid-structure Interaction modelling of patient specific 3D arterial blood flow?

I am getting convergence issues in the first coupling iteration itself and the solver stops. I tried refining the mesh and also tried reducing the time step size. It is still not working. If...

06 July 2020 6,617 7 View

Influence of COVID on the mental health - Survey?

Dear Pls, follow the link provided below to register to invaluable experiences in the face of COVID-19. https://swinuw.au1.qualtrics.com/jfe/form/SV_88qs1TRRQAv8Q9T

14 April 2020 384 3 View

Can anyone say about to make a perfect way to make GO bases Nanocomposite?

I am working in ternary composites. Is there anyone working in graphene and its derivatives. I want to make a composite comprised of GO with some inorganic material.

05 May 2019 6,977 3 View

How to model the leakage of water from a closed container when the valve is opened?

I patched the fluid domain with 1400 gauge pressure and set up the boundary condition at the outlet to be 0 gauge pressure. But I am getting floating point exception. What am i missing?

04 April 2019 8,579 4 View

Can anyone say clearly about the calculation for doping metal oxides using some different mol ratios?

I need to dope metal to my metal oxide matrix, Can anyone clearly explain me about the calculations that i need to workout for mol %.

26 March 2019 1,470 4 View

Is there any medium to support the growth of Streptomycetes only?

Does the growth of microbes on ISP4 medium confirm the organisms to be a streptomycete? Kindly suggest some selection medium for Streptomycetes

17 February 2019 7,336 3 View

Where can can I buy Ceramic Matrix Composites (CMC) SiC-C or SiC-SiC?

Hi, Does any company in Europe sell Ceramic Matrix Composites (CMC) (SiC-C or SiC-SiC) components or flat plates. Thanks Sundar

04 February 2019 4,467 3 View

Reference required - reg?

Dear Friends, In Biodiversity Heritage Library, I couldn't download the volumes from 8 onwards. If anybody is having the following reference, kindly support me to share it. "Clark AM 1967. Notes...

02 January 2019 6,975 6 View

Air pollutant accumulated Filter paper preservation?

Usually the filter papers will be desiccated for 24 hr before and after ambient air pollutant sampling. As we encounter some error in weighing balance, we are unable to weigh the exposed air...

22 October 2018 1,925 4 View

Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

How to calculate CCS for Sodiated adduct ions and Multiply Charged Ions?

I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...

08 August 2024 8,329 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

Is there a good freeware software for photodocumentation of TLC?

I need something like Camag's VisionCat software for photodocumentation of TLCs. Could you recommend something?

31 July 2024 8,415 1 View