Dear Anthony 

Please find specific procedure in attachments for isolation.

Dear Neeraj

But, I dont think that the MeOHCH2Cl2-aqueous NH3 (50:50:1) would be the best solvent. The extraction result of the crude extract  is only: 119 g from 1 kg raw material.

Anyway, thanks you for your kind support

Dear Anthony

Why not you validate your own extraction method, by using successive solvent extraction method.

Dear Neeraj,

Ha..ha.. you are right!

I just want to know whether there are someones else that doing the same study, so that I need not to do the replication on the same subject. Make the unifying, save the time and energy, right!

Dear Anthony

The attached information may be useful in finding an answer to your query.

Good luck.

Dear Dr. Rao,

Thanks a lot for your kind support.

best regards, anthony