What is the optimal seeding density and concentration of IncuCyte® NucLight Rapid Red for U-2 OS cell proliferation assay?

06 November 2019 1 6K Report

I plan to assay proliferation dynamics of U-2 OS cells using the IncuCyte platform in a 96 well format. I will be using the IncuCyte NucLight Rapid Red (

https://eu-shop.essenbioscience.com/collections/reagents/products/nuclight-rapid-red-reagent-for-live-cell-nuclear-labeling) for labeling. If you have any experience using this dye for this cell line, what do you think is the optimal seeding density and dye concentration if I plan to record for 5-6 days?

Mohammad Tariq Ali Khan

5000 cells per well in 100uL media

make it 500000 cells in 10mL

per mL will be 50k

100uL 5000

also depends on treat time

if 72 h.. seed only 2500 cells per well.

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