KL Nikhil

6 Questions 17 Answers 0 Followers

Questions related from KL Nikhil

What is the optimal seeding density and concentration of IncuCyte® NucLight Rapid Red for U-2 OS cell proliferation assay?

I plan to assay proliferation dynamics of U-2 OS cells using the IncuCyte platform in a 96 well format. I will be using the IncuCyte NucLight Rapid Red (...

06 November 2019 5,710 1 View

Is it possible to extract coupling function from time series data of a population of coupled oscillators ?

I have two sets of oscillator populations A and B with different frequencies and amplitudes; for instance population A has higher frequency and lower amplitude while population B has lower...

03 January 2019 7,981 6 View

In a network of diffusive coupled oscillators how does coupling strength change as a function of concentration of coupling factor/agent?

In a network of coupled oscillators (diffusive coupling) while it is intuitive to expect that the magnitude of coupling increases with increase in concentration of coupling factor (provided the...

28 November 2015 7,410 7 View

Recombinant Inbred Lines(RILs) versus Near Isogenic Lines (NILs)

If one is interested in QTL analysis of laboratory out-bred Drosophila populations, what are the pros and cons of using Recombinant Inbred Lines (RILs) and Near Isogenic Lines (NILs) or is there...

18 February 2013 2,825 12 View

How to avoid Drosophila isofemale lines from crashing?

I need to generate isofemale lines of Drosophila melanogaster but the last time we initiated a set of such lines, most of the lines crashed possibly due to inbreeding depression. I am aware that...

23 November 2012 9,103 5 View

What is the average efficiency of PCR reactions in general? (FYI: I use qPCR with sybr green chemistry in ABI Step One plus machine)

Ideally the efficiency of a PCR should be 100% and qPCR tutorials online say that it should be somewhere between 90% to 100 %. In one of the recent talks that I attended, I was told that if my PCR...

12 November 2012 5,229 15 View