Hi, I will be studying the effects of organic arsenic exposure on rat's long bone for my master's degree and I am planning to use the right femur to measure the lipid peroxidation status (malondialdehyde level) and antioxidant enzymes (glutathione peroxidase and superoxide dismutase). I have found a method that requires the femur to be snap frozen at -80 degree Celcius and homogenised by grinding using pestle and mortar. I am a little bit unsure about the part where MDA and oxidative enzymes are measure. Would anyone be able to tell me the optimal method for doing these tests? I was also wondering if there were any other methods of obtaining the bone homogenates? You are more than welcome to share your research experience here. Thank you!
Abstract
As an initial subdeficient status of zinc, considered as an essential antioxidant trace element, is frequent in burned patients, we aim to assess the effects of low zinc dietary intakes on burn induced oxidative stress, in an animal model. After eight weeks of conditioning diets containing 80 ppm (control group) or 10 ppm of zinc (depleted group), Wistar rats were 20% TBSA burned and sampled one to ten days after injury. Kinetic evolutions of zinc status, plasma oxidative stress parameters and antioxidant enzymes were also studied in blood and organs.
As an initial subdeficient status of zinc, considered as an essential antioxidant trace element, is frequent in burned patients, we aim to assess the effects of low zinc dietary intakes on burn induced oxidative stress, in an animal model. After eight weeks of conditioning diets containing 80 ppm (control group) or 10 ppm of zinc (depleted group), Wistar rats were 20% TBSA burned and sampled one to ten days after injury. Kinetic evolutions of zinc status, plasma oxidative stress parameters and antioxidant enzymes were also studied in blood and organs.
As an initial subdeficient status of zinc, considered as an essential antioxidant trace element, is frequent in burned patients, we aim to assess the effects of low zinc dietary intakes on burn induced oxidative stress, in an animal model. After eight weeks of conditioning diets containing 80 ppm (control group) or 10 ppm of zinc (depleted group), Wistar rats were 20% TBSA burned and sampled one to ten days after injury. Kinetic evolutions of zinc status, plasma oxidative stress parameters and antioxidant enzymes were also studied in blood and organs.
As an initial subdeficient status of zinc, considered as an essential antioxidant trace element, is frequent in burned patients, we aim to assess the effects of low zinc dietary intakes on burn induced oxidative stress, in an animal model. After eight weeks of conditioning diets containing 80 ppm (control group) or 10 ppm of zinc (depleted group), Wistar rats were 20% TBSA burned and sampled one to ten days after injury. Kinetic evolutions of zinc status, plasma oxidative stress parameters and antioxidant enzymes were also studied in blood and organs.
The zinc depleted diet induced, before injury, a significant decrease in zinc bone level and the increase of oxidative stress markers without stimulation of antioxidant enzyme activity. After burn, more markedly in zinc depleted animals than in controls, zinc levels decreased in plasma and bone, while increasing in liver. The decrease of thiol groups and GSH/GSSG ratio and the depression of GPx activity in liver are also moderately emphasized.
Nevertheless, depleted zinc status could not be considered as determining for oxidative damages after burn injury. Further investigations must also be done to enlighten the mechanism of beneficial effects of zinc supplementation reported in burned patients.
Keywords: Animal Feed, Animals, Burns, metabolism, physiopathology, Diet, Dietary Supplements, Gluconates, administration & dosage, pharmacology, Male, Oxidative Stress, drug effects, Rats, Rats, Wistar, Thiobarbituric Acid Reactive Substances, metabolism, Tissue Distribution.
Article Burn-induced Oxidative Stress is Altered by a Low Zinc Statu...
Just a suggestion, maybe you can also extract the mRNA from measure the mRNA level of glutathione peroxidase and superoxide dismutase to see whether they are changed.
That is a great idea, Yuanyuan Liu. Thank you for the suggestion!
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